Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support

T. Shioyama, A. Suzuki, K. Nomura, N. Shigaki, H. Kubo, S. Takeda, S. Yamamori, T. Maruyama, Hiroshi Iseki, T. Komori, M. Nitta, T. Sakayori, Y. Muragaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Frozen section studies are a useful method to rapidly define tumor malignancy and identify the extent of surgical resection. However, diagnosis with a frozen section is qualitative and sometimes difficult. Therefore a quantitative method for grading tumors is desired. We have already reported a technique of intraoperative flow cytometry (iFC) that supports intraoperative histopathological examination of frozen sections. In this study, we report an advanced system named 'Fully Automatic Rapid DNA Ploidy Analyzer' with a tissue pretreatment function and a freeze-dried reagent kit for cell staining. To evaluate our system, we analyzed samples from glioma patients who underwent open surgery for brain tumors. We observed obvious difference of the Malignancy Index (MI) between neoplastic and perilesional brain tissue (26.0 ±22.1% and 4.1 ±2.5%, respectively, P<0.001). Cut-off level for identification of the tumor in the biopsy specimen was 6.8% which provided 86% sensitivity and 81% specificity. We also obtained a good correlation between the MI and histological grade (WHO grading). Our new system also enabled finishing the process from sample preparation to the end of analysis in ten minutes or less. These results demonstrate that our fully automatic rapid DNA ploidy analyzer is feasible for rapid determination of glioma presence in a surgical biopsy sample.

Original languageEnglish
Title of host publicationProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Pages906-909
Number of pages4
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka
Duration: 2013 Jul 32013 Jul 7

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CityOsaka
Period13/7/313/7/7

Fingerprint

Ploidies
Tumors
DNA
Frozen Sections
Biopsy
Brain
Neoplasms
Glioma
Tissue
Flow cytometry
Surgery
Neoplasm Grading
Brain Neoplasms
Flow Cytometry
Staining and Labeling
Sensitivity and Specificity

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

Shioyama, T., Suzuki, A., Nomura, K., Shigaki, N., Kubo, H., Takeda, S., ... Muragaki, Y. (2013). Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 906-909). [6609648] https://doi.org/10.1109/EMBC.2013.6609648

Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support. / Shioyama, T.; Suzuki, A.; Nomura, K.; Shigaki, N.; Kubo, H.; Takeda, S.; Yamamori, S.; Maruyama, T.; Iseki, Hiroshi; Komori, T.; Nitta, M.; Sakayori, T.; Muragaki, Y.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 906-909 6609648.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shioyama, T, Suzuki, A, Nomura, K, Shigaki, N, Kubo, H, Takeda, S, Yamamori, S, Maruyama, T, Iseki, H, Komori, T, Nitta, M, Sakayori, T & Muragaki, Y 2013, Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support. in Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6609648, pp. 906-909, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, 13/7/3. https://doi.org/10.1109/EMBC.2013.6609648
Shioyama T, Suzuki A, Nomura K, Shigaki N, Kubo H, Takeda S et al. Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support. In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 906-909. 6609648 https://doi.org/10.1109/EMBC.2013.6609648
Shioyama, T. ; Suzuki, A. ; Nomura, K. ; Shigaki, N. ; Kubo, H. ; Takeda, S. ; Yamamori, S. ; Maruyama, T. ; Iseki, Hiroshi ; Komori, T. ; Nitta, M. ; Sakayori, T. ; Muragaki, Y. / Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. pp. 906-909
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