Functional analysis of lysosomes during mouse preimplantation embryo development

Satoshi Tsukamoto, Taichi Hara, Atsushi Yamamoto, Yuki Ohta, Ayako Wada, Yuka Ishida, Seiji Kito, Tetsu Nishikawa, Naojiro Minami, Ken Sato, Toshiaki Kokubo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our fndings highlight the functional changes in lysosomes in mouse preimplantation embryos.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Reproduction and Development
Volume59
Issue number1
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

lysosomes
embryogenesis
mice
morula
blastocyst
embryo (animal)
cathepsin D
cathepsin B
cells
membrane proteins
organelles
protein synthesis
degradation

Keywords

  • Cathepsin
  • Lipofuscin
  • Lysosome
  • Lysosome-associated membrane protein (LAMP)
  • Preimplantaion embryo

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Tsukamoto, S., Hara, T., Yamamoto, A., Ohta, Y., Wada, A., Ishida, Y., ... Kokubo, T. (2013). Functional analysis of lysosomes during mouse preimplantation embryo development. Journal of Reproduction and Development, 59(1), 33-39.

Functional analysis of lysosomes during mouse preimplantation embryo development. / Tsukamoto, Satoshi; Hara, Taichi; Yamamoto, Atsushi; Ohta, Yuki; Wada, Ayako; Ishida, Yuka; Kito, Seiji; Nishikawa, Tetsu; Minami, Naojiro; Sato, Ken; Kokubo, Toshiaki.

In: Journal of Reproduction and Development, Vol. 59, No. 1, 2013, p. 33-39.

Research output: Contribution to journalArticle

Tsukamoto, S, Hara, T, Yamamoto, A, Ohta, Y, Wada, A, Ishida, Y, Kito, S, Nishikawa, T, Minami, N, Sato, K & Kokubo, T 2013, 'Functional analysis of lysosomes during mouse preimplantation embryo development', Journal of Reproduction and Development, vol. 59, no. 1, pp. 33-39.
Tsukamoto, Satoshi ; Hara, Taichi ; Yamamoto, Atsushi ; Ohta, Yuki ; Wada, Ayako ; Ishida, Yuka ; Kito, Seiji ; Nishikawa, Tetsu ; Minami, Naojiro ; Sato, Ken ; Kokubo, Toshiaki. / Functional analysis of lysosomes during mouse preimplantation embryo development. In: Journal of Reproduction and Development. 2013 ; Vol. 59, No. 1. pp. 33-39.
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