Functional analysis of two isoforms of leaf-type ferredoxin-NADP +-oxidoreductase in rice using the heterologous expression system of arabidopsis

Mieko Higuchi-Takeuchi, Takanari Ichikawa, Youichi Kondou, Keiko Matsui, Yukako Hasegawa, Mika Kawashima, Kintake Sonoike, Masaki Mori, Hirohiko Hirochika, Minami Matsui

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Ferredoxin-NADP +-oxidoreductase (FNR) mediates electron transfer between ferredoxin (Fd) and NADP +; therefore, it is a key enzyme that provides the reducing power used in the Calvin cycle. Other than FNR, nitrite reductase, sulfite reductase, glutamate synthase, and Fd-thioredoxin reductase also accept electrons from Fd, an electron carrier protein in the stroma. Therefore, the regulation of electron partitioning in the chloroplast is important for photosynthesis and other metabolic pathways. The regulatory mechanism of electron partitioning, however, remains to be elucidated. We found, by taking advantage of a gain-of-function approach, that expression of two rice (Oryza sativa) full-length cDNAs of leaf-type FNRs (OsLFNR1 and OsLFNR2) led to altered chlorophyll fluorescence and growth in Arabidopsis (Arabidopsis thaliana) and rice. We revealed that overexpression of the OsLFNR1 and OsLFNR2 full-length cDNAs resulted in distinct phenotypes despite the high sequence similarity between them. Expression of OsLFNR1 affected the nitrogen assimilation pathway without inhibition of photosynthesis under normal conditions. On the other hand, OsLFNR2 expression led to the impairment of photosynthetic linear electron transport as well as Fd-dependent cyclic electron flow around photosystem I. The endogenous protein level of OsLFNR was found to be suppressed in both OsLFNR1- and OsLFNR2-overexpressing rice plants, leading to changes in the stoichiometry of the two LFNR isoforms within the thylakoid and soluble fractions. Thus, we propose that the stoichiometry of two LFNR isoforms plays an important role in electron partitioning between carbon fixation and nitrogen assimilation.

    Original languageEnglish
    Pages (from-to)96-108
    Number of pages13
    JournalPlant Physiology
    Volume157
    Issue number1
    DOIs
    Publication statusPublished - 2011 Sep

    Fingerprint

    Ferredoxin-NADP Reductase
    ferredoxin-NADP reductase
    Arabidopsis
    Protein Isoforms
    ferredoxins
    electrons
    Electrons
    rice
    Ferredoxins
    Photosynthesis
    leaves
    Calvin cycle
    stoichiometry
    electron transfer
    assimilation (physiology)
    glutamate synthase (ferredoxin)
    photosynthesis
    Oxidoreductases Acting on Sulfur Group Donors
    Nitrogen
    Complementary DNA

    ASJC Scopus subject areas

    • Plant Science
    • Genetics
    • Physiology

    Cite this

    Higuchi-Takeuchi, M., Ichikawa, T., Kondou, Y., Matsui, K., Hasegawa, Y., Kawashima, M., ... Matsui, M. (2011). Functional analysis of two isoforms of leaf-type ferredoxin-NADP +-oxidoreductase in rice using the heterologous expression system of arabidopsis. Plant Physiology, 157(1), 96-108. https://doi.org/10.1104/pp.111.181248

    Functional analysis of two isoforms of leaf-type ferredoxin-NADP +-oxidoreductase in rice using the heterologous expression system of arabidopsis. / Higuchi-Takeuchi, Mieko; Ichikawa, Takanari; Kondou, Youichi; Matsui, Keiko; Hasegawa, Yukako; Kawashima, Mika; Sonoike, Kintake; Mori, Masaki; Hirochika, Hirohiko; Matsui, Minami.

    In: Plant Physiology, Vol. 157, No. 1, 09.2011, p. 96-108.

    Research output: Contribution to journalArticle

    Higuchi-Takeuchi, M, Ichikawa, T, Kondou, Y, Matsui, K, Hasegawa, Y, Kawashima, M, Sonoike, K, Mori, M, Hirochika, H & Matsui, M 2011, 'Functional analysis of two isoforms of leaf-type ferredoxin-NADP +-oxidoreductase in rice using the heterologous expression system of arabidopsis', Plant Physiology, vol. 157, no. 1, pp. 96-108. https://doi.org/10.1104/pp.111.181248
    Higuchi-Takeuchi, Mieko ; Ichikawa, Takanari ; Kondou, Youichi ; Matsui, Keiko ; Hasegawa, Yukako ; Kawashima, Mika ; Sonoike, Kintake ; Mori, Masaki ; Hirochika, Hirohiko ; Matsui, Minami. / Functional analysis of two isoforms of leaf-type ferredoxin-NADP +-oxidoreductase in rice using the heterologous expression system of arabidopsis. In: Plant Physiology. 2011 ; Vol. 157, No. 1. pp. 96-108.
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