Functional interaction of regulatory factors with the Pgc-1α promoter in response to exercise by in vivo imaging

Takayuki Akimoto, Ping Li, Zhen Yan

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Real-time optical bioluminescence imaging is a powerful tool for studies of gene regulation in living animals. To elucidate exercise-induced signaling/transcriptional control of the peroxisome proliferator-activated receptor-γ coactivator-1α (Pgc-1α) gene in skeletal muscle, we combined this technology with electric pulse-mediated gene transfer to cotransfect the Pgc-1α reporter gene with plasmid DNA encoding mutant/deletion forms of putative regulatory factors and, thereby, assess the responsiveness of the promoter to skeletal muscle contraction. We show that each of the myocyte enhancer factor 2 sites on the Pgc-1α promoter is required for contractile activity-induced Pgc-1α transcription. The responsiveness of the Pgc-1α promoter to contractile activity could be completely blocked by overexpression of the dominant-negative form of activating transcription factor 2 (ATF2), the signaling-resistant form of histone deacetylase (HDAC) 5 (HDAC5), or protein kinase D (PKD), but not by HDAC4. These findings provide in vivo evidence for functional interactions between PKD/HDAC5 and ATF2 regulatory factors and the Pgc-1α gene in adult skeletal muscle.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume295
Issue number1
DOIs
Publication statusPublished - 2008 Jul
Externally publishedYes

Fingerprint

Activating Transcription Factor 2
Skeletal Muscle
Genes
MEF2 Transcription Factors
Peroxisome Proliferator-Activated Receptors
Histone Deacetylases
Optical Imaging
Muscle Contraction
Reporter Genes
Plasmids
Technology
DNA
protein kinase D

Keywords

  • Electric pulse-mediated gene transfer
  • Optical biolunminescence imaging
  • Reporter gene
  • Signal transduction
  • Transcriptional control

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this

@article{251dcae6398e40d6a3c904df806e85a7,
title = "Functional interaction of regulatory factors with the Pgc-1α promoter in response to exercise by in vivo imaging",
abstract = "Real-time optical bioluminescence imaging is a powerful tool for studies of gene regulation in living animals. To elucidate exercise-induced signaling/transcriptional control of the peroxisome proliferator-activated receptor-γ coactivator-1α (Pgc-1α) gene in skeletal muscle, we combined this technology with electric pulse-mediated gene transfer to cotransfect the Pgc-1α reporter gene with plasmid DNA encoding mutant/deletion forms of putative regulatory factors and, thereby, assess the responsiveness of the promoter to skeletal muscle contraction. We show that each of the myocyte enhancer factor 2 sites on the Pgc-1α promoter is required for contractile activity-induced Pgc-1α transcription. The responsiveness of the Pgc-1α promoter to contractile activity could be completely blocked by overexpression of the dominant-negative form of activating transcription factor 2 (ATF2), the signaling-resistant form of histone deacetylase (HDAC) 5 (HDAC5), or protein kinase D (PKD), but not by HDAC4. These findings provide in vivo evidence for functional interactions between PKD/HDAC5 and ATF2 regulatory factors and the Pgc-1α gene in adult skeletal muscle.",
keywords = "Electric pulse-mediated gene transfer, Optical biolunminescence imaging, Reporter gene, Signal transduction, Transcriptional control",
author = "Takayuki Akimoto and Ping Li and Zhen Yan",
year = "2008",
month = "7",
doi = "10.1152/ajpcell.00104.2008",
language = "English",
volume = "295",
journal = "American Journal of Physiology - Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Functional interaction of regulatory factors with the Pgc-1α promoter in response to exercise by in vivo imaging

AU - Akimoto, Takayuki

AU - Li, Ping

AU - Yan, Zhen

PY - 2008/7

Y1 - 2008/7

N2 - Real-time optical bioluminescence imaging is a powerful tool for studies of gene regulation in living animals. To elucidate exercise-induced signaling/transcriptional control of the peroxisome proliferator-activated receptor-γ coactivator-1α (Pgc-1α) gene in skeletal muscle, we combined this technology with electric pulse-mediated gene transfer to cotransfect the Pgc-1α reporter gene with plasmid DNA encoding mutant/deletion forms of putative regulatory factors and, thereby, assess the responsiveness of the promoter to skeletal muscle contraction. We show that each of the myocyte enhancer factor 2 sites on the Pgc-1α promoter is required for contractile activity-induced Pgc-1α transcription. The responsiveness of the Pgc-1α promoter to contractile activity could be completely blocked by overexpression of the dominant-negative form of activating transcription factor 2 (ATF2), the signaling-resistant form of histone deacetylase (HDAC) 5 (HDAC5), or protein kinase D (PKD), but not by HDAC4. These findings provide in vivo evidence for functional interactions between PKD/HDAC5 and ATF2 regulatory factors and the Pgc-1α gene in adult skeletal muscle.

AB - Real-time optical bioluminescence imaging is a powerful tool for studies of gene regulation in living animals. To elucidate exercise-induced signaling/transcriptional control of the peroxisome proliferator-activated receptor-γ coactivator-1α (Pgc-1α) gene in skeletal muscle, we combined this technology with electric pulse-mediated gene transfer to cotransfect the Pgc-1α reporter gene with plasmid DNA encoding mutant/deletion forms of putative regulatory factors and, thereby, assess the responsiveness of the promoter to skeletal muscle contraction. We show that each of the myocyte enhancer factor 2 sites on the Pgc-1α promoter is required for contractile activity-induced Pgc-1α transcription. The responsiveness of the Pgc-1α promoter to contractile activity could be completely blocked by overexpression of the dominant-negative form of activating transcription factor 2 (ATF2), the signaling-resistant form of histone deacetylase (HDAC) 5 (HDAC5), or protein kinase D (PKD), but not by HDAC4. These findings provide in vivo evidence for functional interactions between PKD/HDAC5 and ATF2 regulatory factors and the Pgc-1α gene in adult skeletal muscle.

KW - Electric pulse-mediated gene transfer

KW - Optical biolunminescence imaging

KW - Reporter gene

KW - Signal transduction

KW - Transcriptional control

UR - http://www.scopus.com/inward/record.url?scp=52749095883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52749095883&partnerID=8YFLogxK

U2 - 10.1152/ajpcell.00104.2008

DO - 10.1152/ajpcell.00104.2008

M3 - Article

C2 - 18434626

AN - SCOPUS:52749095883

VL - 295

JO - American Journal of Physiology - Cell Physiology

JF - American Journal of Physiology - Cell Physiology

SN - 0363-6143

IS - 1

ER -