Functionalization of ultradispersed diamond for DNA detection

Jung Hoon Yang; Yoshikazu Nakano; Yasunori Murakami; Kwang Soup Song; Hiroshi Kawarada

doi:10.1007/s11051-008-9367-2

Functionalization of ultradispersed diamond for DNA detection

Jung Hoon Yang^*, Yoshikazu Nakano, Yasunori Murakami, Kwang Soup Song, Hiroshi Kawarada

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

Original language	English
Pages (from-to)	69-75
Number of pages	7
Journal	Journal of Nanoparticle Research
Volume	10
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1007/s11051-008-9367-2
Publication status	Published - 2008 Dec

Keywords

Amination
Carboxylation
DNA immobilization
Functionalization
Nanomedicine
Ultradispersed diamond

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
Chemistry(all)
Modelling and Simulation
Materials Science(all)
Condensed Matter Physics

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10.1007/s11051-008-9367-2

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title = "Functionalization of ultradispersed diamond for DNA detection",

abstract = "Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.",

keywords = "Amination, Carboxylation, DNA immobilization, Functionalization, Nanomedicine, Ultradispersed diamond",

author = "Yang, {Jung Hoon} and Yoshikazu Nakano and Yasunori Murakami and Song, {Kwang Soup} and Hiroshi Kawarada",

note = "Funding Information: Acknowledgments This work was carried out in the Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW). This work is also supported in part by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology.",

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doi = "10.1007/s11051-008-9367-2",

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AU - Yang, Jung Hoon

AU - Nakano, Yoshikazu

AU - Murakami, Yasunori

AU - Song, Kwang Soup

AU - Kawarada, Hiroshi

N1 - Funding Information: Acknowledgments This work was carried out in the Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW). This work is also supported in part by a Grant-in-Aid for Center of Excellence (COE) Research from the Ministry of Education, Culture, Sports, Science and Technology.

PY - 2008/12

Y1 - 2008/12

N2 - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

AB - Functionalization of nanoparticles and immobilization of biomolecules on nanoparticles are important techniques for various applications in biotechnology. For application in the biomedical field, the surface of ultradispersed diamond (UDD) is immobilized with amino-terminated DNA after functionalizing carboxylic or amino group on the UDD surface. The characterized surface of UDD, carboxylated UDD, and aminated UDD were analyzed by DRIFT spectroscopy. In addition, hybridization with Cy-5 labeled target DNA was clearly observed on functionalized UDD by fluorescence microscopy.

KW - Amination

KW - Carboxylation

KW - DNA immobilization

KW - Functionalization

KW - Nanomedicine

KW - Ultradispersed diamond

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Functionalization of ultradispersed diamond for DNA detection

Abstract

Keywords

ASJC Scopus subject areas

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