Functionalized nanoparticles for measurement of biomarkers using a SERS nanochannel platform

Melodie Benford*, Miao Wang, Jun Kameoka, Theresa Good, Gerard Cote

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The overall goal of this research is to develop a new point-of-care system for early detection and characterization of cardiac markers to aid in diagnosis of acute coronary syndrome. The envisioned final technology platform incorporates functionalized gold colloidal nanoparticles trapped at the entrance to a nanofluidic device providing a robust means for analyte detection at trace levels using surface enhanced Raman spectroscopy (SERS). To discriminate a specific biomarker, we designed an assay format analogous to a competitive ELISA. Notably, the biomarker would be captured by an antibody and in turn displace a peptide fragment, containing the binding epitope of the antibody labeled with a Raman reporter molecule that would not interfere with blood serum proteins. To demonstrate the feasibility of this approach, we used C-reactive protein (CRP) as a surrogate biomarker. We functionalized agarose beads with anti-CRP that were placed outside the nanochannel, then added either Rhodamine-6-G (R6G) labeled-CRP and gold (as a surrogate of a sample without analyte present), or R6G labeled CRP, gold, and unlabeled CRP (as a surrogate of a sample with analyte present). Analyzing the spectra we see an increase in peak intensity in the presence of analyte at characteristic peaks for R6G specifically, 1284 and1567 cm- 1. Further, our results illustrate the reproducibility of the Raman spectra collected for R6G-labeled CRP in the nanochannel. Overall, we believe that this method will provide the advantage of sensitivity and narrow line widths characteristic of SERS as well as the specificity toward the biomarker of interest.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine VII
DOIs
Publication statusPublished - 2010
Externally publishedYes
EventPlasmonics in Biology and Medicine VII - San Francisco, CA, United States
Duration: 2010 Jan 252010 Jan 28

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7577
ISSN (Print)1605-7422

Conference

ConferencePlasmonics in Biology and Medicine VII
Country/TerritoryUnited States
CitySan Francisco, CA
Period10/1/2510/1/28

Keywords

  • C-reactive protein
  • Cardiac biomarker
  • Colloidal gold
  • Competitive immunoassay
  • Nanochannel
  • Nanofluidic device
  • Point-of-care diagnostics
  • Raman spectroscopy
  • SERS
  • Surface enhanced

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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