Functionalized three-dimensional carbon microarrays for cancer biomarker detection

V. Penmatsa, A. R. Ruslinda, M. Beidaghi, H. Kawarada, C. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

A label-free detection strategy for the detection of platelet-derived growth factor (PDGF-BB) oncoprotein detection using signaling aptamer/protein binding complex is reported. The 3D carbon microarrays detection platform was fabricated by pyrolyzing patterned photoresist and surface functionalized using directamination technique. The detection strategy is based on the release of TOTO intercalating dye from the target binding aptamer's stem structure when it captures PDGF. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration with a very good detection limit of 5 pmol. This detection strategy is promising for the potential detection of different cancer biomarkers and proteins.

Original languageEnglish
Title of host publicationNano/Bio Sensors
Pages7-14
Number of pages8
Edition15
DOIs
Publication statusPublished - 2013 Oct 22
EventSymposium on Nano/Bio Sensors - 221st ECS Meeting - Seattle, WA, United States
Duration: 2012 May 62012 May 10

Publication series

NameECS Transactions
Number15
Volume45
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

ConferenceSymposium on Nano/Bio Sensors - 221st ECS Meeting
CountryUnited States
CitySeattle, WA
Period12/5/612/5/10

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Penmatsa, V., Ruslinda, A. R., Beidaghi, M., Kawarada, H., & Wang, C. (2013). Functionalized three-dimensional carbon microarrays for cancer biomarker detection. In Nano/Bio Sensors (15 ed., pp. 7-14). (ECS Transactions; Vol. 45, No. 15). https://doi.org/10.1149/04515.0007ecst