Functionalized three-dimensional carbon microarrays for cancer biomarker detection

V. Penmatsa, A. R. Ruslinda, M. Beidaghi, Hiroshi Kawarada, C. Wang

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    8 Citations (Scopus)

    Abstract

    A label-free detection strategy for the detection of platelet-derived growth factor (PDGF-BB) oncoprotein detection using signaling aptamer/protein binding complex is reported. The 3D carbon microarrays detection platform was fabricated by pyrolyzing patterned photoresist and surface functionalized using directamination technique. The detection strategy is based on the release of TOTO intercalating dye from the target binding aptamer's stem structure when it captures PDGF. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration with a very good detection limit of 5 pmol. This detection strategy is promising for the potential detection of different cancer biomarkers and proteins.

    Original languageEnglish
    Title of host publicationECS Transactions
    Pages7-14
    Number of pages8
    Volume45
    Edition15
    DOIs
    Publication statusPublished - 2013
    EventSymposium on Nano/Bio Sensors - 221st ECS Meeting - Seattle, WA
    Duration: 2012 May 62012 May 10

    Other

    OtherSymposium on Nano/Bio Sensors - 221st ECS Meeting
    CitySeattle, WA
    Period12/5/612/5/10

    Fingerprint

    Microarrays
    Proteins
    Carbon
    Photoresists
    Platelets
    Labels
    Dyes
    Fluorescence
    Sensors
    Tumor Biomarkers
    Intercellular Signaling Peptides and Proteins
    Protein Binding

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Penmatsa, V., Ruslinda, A. R., Beidaghi, M., Kawarada, H., & Wang, C. (2013). Functionalized three-dimensional carbon microarrays for cancer biomarker detection. In ECS Transactions (15 ed., Vol. 45, pp. 7-14) https://doi.org/10.1149/04515.0007ecst

    Functionalized three-dimensional carbon microarrays for cancer biomarker detection. / Penmatsa, V.; Ruslinda, A. R.; Beidaghi, M.; Kawarada, Hiroshi; Wang, C.

    ECS Transactions. Vol. 45 15. ed. 2013. p. 7-14.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Penmatsa, V, Ruslinda, AR, Beidaghi, M, Kawarada, H & Wang, C 2013, Functionalized three-dimensional carbon microarrays for cancer biomarker detection. in ECS Transactions. 15 edn, vol. 45, pp. 7-14, Symposium on Nano/Bio Sensors - 221st ECS Meeting, Seattle, WA, 12/5/6. https://doi.org/10.1149/04515.0007ecst
    Penmatsa V, Ruslinda AR, Beidaghi M, Kawarada H, Wang C. Functionalized three-dimensional carbon microarrays for cancer biomarker detection. In ECS Transactions. 15 ed. Vol. 45. 2013. p. 7-14 https://doi.org/10.1149/04515.0007ecst
    Penmatsa, V. ; Ruslinda, A. R. ; Beidaghi, M. ; Kawarada, Hiroshi ; Wang, C. / Functionalized three-dimensional carbon microarrays for cancer biomarker detection. ECS Transactions. Vol. 45 15. ed. 2013. pp. 7-14
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