Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site- directed mutagenesis

Satoshi Akanuma, Akihiko Yamagishi, Nobuo Tanaka, Tairo Oshima

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A thermostabilized mutant of Bacillus subtilis 3-isopropylmalate dehydrogenase (IPMDH) obtained in a previous study contained a set of triple amino acid substitutions. To further improve the stability of the mutant, we used a random mutagenesis technique and identified two additional thermostabilizing substitutions, Thr22→Lys and Met256→Val, that separately endowed the protein with further stability. We introduced the two mutations into a single enzyme molecule, thus constructing a mutant with overall quintuple mutations. Other studies have suggested that an improved hydrophobic subunit interaction and a rigid type II β-turn play important roles in enhancing the protein stability. Based on those observations, we successively introduced amino acid substitutions into the mutant with the quintuple mutations by site-directed mutagenesis: Glu253 at the subunit interface was replaced by Leu to increase the hydrophobic interaction between the subunits; Glu112, Ser113 and Ser115 that were involved in the formation of the turn were replaced by Pro, Gly and Glu, respectively, to make the turn more rigid. The thermal stability of the mutants was determined based on remaining activity after heat treatment and first-order rate constant of thermal unfolding, which showed gradual increases in thermal stability as more mutations were included.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalEuropean Journal of Biochemistry
Volume260
Issue number2
DOIs
Publication statusPublished - 1999 Mar 1
Externally publishedYes

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3-Isopropylmalate Dehydrogenase
Mutagenesis
Bacilli
Site-Directed Mutagenesis
Bacillus subtilis
Thermodynamic stability
Substitution reactions
Hot Temperature
Mutation
Amino Acid Substitution
Hydrophobic and Hydrophilic Interactions
Amino Acids
Protein Stability
Rate constants
Proteins
Heat treatment
Molecules
Enzymes

Keywords

  • 3-isopropylmalate dehydrogenase
  • Random mutagenesis
  • Site-directed mutagenesis
  • Thermal stability
  • Thermus thermophilus

ASJC Scopus subject areas

  • Biochemistry

Cite this

Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site- directed mutagenesis. / Akanuma, Satoshi; Yamagishi, Akihiko; Tanaka, Nobuo; Oshima, Tairo.

In: European Journal of Biochemistry, Vol. 260, No. 2, 01.03.1999, p. 499-504.

Research output: Contribution to journalArticle

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