Fusaramin, an antimitochondrial compound produced by Fusarium sp., discovered using multidrug-sensitive Saccharomyces cerevisiae

Katsuyuki Sakai, Yufu Unten, Masato Iwatsuki, Hirotaka Matsuo, Wataru Fukasawa, Tomoyasu Hirose, Takumi Chinen, Kenichi Nonaka, Takuji Nakashima, Toshiaki Sunazuka, Takeo Usui, Masatoshi Murai, Hideto Miyoshi, Yukihiro Asami, Satoshi Ōmura, Kazuro Shiomi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A new compound, fusaramin (1), along with three known compounds, sambutoxin (2), N-demethylsambutoxin (3) and (−)-6-deoxyoxysporidinone (4), was isolated from a culture broth of Fusarium sp. FKI-7550 by bioassay-guided fractionation using multidrug-sensitive Saccharomyces cerevisiae 12geneΔ0HSR-iERG6. The chemical structure of 1 was elucidated by NMR studies and electronic circular dichroism spectrum. Compound 1 showed antibacterial activity against some Gram-positive and Gram-negative bacteria and inhibited the growth of S. cerevisiae 12geneΔ0HSR-iERG6 grown on glycerol-containing medium. The MICs of 1 against wild-type and multidrug-sensitive yeasts grown on glycerol-containing medium were >128 μg ml−1 and 0.64 μg ml−1, respectively. However, MICs of 1 against both yeast strains grown on glucose-containing medium were >128 μg ml−1. All compounds showed inhibition of ATP synthesis via oxidative phosphorylation using isolated S. cerevisiae mitochondria.

Original languageEnglish
Pages (from-to)645-652
Number of pages8
JournalJournal of Antibiotics
Volume72
Issue number9
DOIs
Publication statusPublished - 2019 Sep 1
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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