Ganglioside composition of GH3 cells: Enhancement of fucoganglioside expression by estradiol, epidermal growth factor and insulin

Shigeo Takamori, Saki Itonori, Kyoko Nakamura, Minoru Suzuki, Akemi Suzuki, Fuyuhiko Inagaki, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The GH3 cell line, a bipotential cell line secreting both prolactin (PRL) and growth hormone (GH), is a useful model for investigating GH/PRL cell lineage differentiation and anterior pituitary adenoma formation. In this study, we investigated the ganglioside composition of GH3 cells and identified two fucogangliosides as the major gangliosides expressed by these cells. Analyses by DEAE-Sephadex A-25 and thin-layer chromatography (TLC) revealed that the GH3 cells contained two major gangliosides, designated FG1 and FG2, respectively. Their structures were identified by fast atom bombardment mass spectrometry and proton nuclear magnetic resonance spectrometry: FG1 is IV2Fucα,II3NeuAc-GgOse4Cer and FG2 is IV2Fucα,IV3Galα,II3NeuAc-GgOse4Cer. Expression of these fucogangliosides was enhanced by chronic treatment with 17β-estradiol (1 nM), epidermal growth factor (10 nM) and insulin (300 nM), which induced differentiation of GH3 cells to normal PRL-secreting cells. Interestingly, immunocytochemistry and flow cytometry revealed that the increased expression of these gangliosides reflected a quantitative change inside the cells but not on the cell surface. These results suggest that the intracellular distribution of fucogangliosides is closely related to the differentiation of GH3 cells.

Original languageEnglish
Pages (from-to)304-314
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1401
Issue number3
DOIs
Publication statusPublished - 1998 Mar 5
Externally publishedYes

Fingerprint

Gangliosides
Epidermal Growth Factor
Estradiol
Insulin
Prolactin
Cell Differentiation
Growth Hormone
Fast Atom Bombardment Mass Spectrometry
Cell Line
Pituitary Neoplasms
Cell Lineage
Thin Layer Chromatography
fucogangliosides
Protons
Spectrum Analysis
Flow Cytometry
Magnetic Resonance Spectroscopy
Immunohistochemistry

Keywords

  • Differentiation
  • Ganglioside
  • GH cell
  • Prolactin

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Ganglioside composition of GH3 cells : Enhancement of fucoganglioside expression by estradiol, epidermal growth factor and insulin. / Takamori, Shigeo; Itonori, Saki; Nakamura, Kyoko; Suzuki, Minoru; Suzuki, Akemi; Inagaki, Fuyuhiko; Shiota, Kunio; Ogawa, Tomoya.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1401, No. 3, 05.03.1998, p. 304-314.

Research output: Contribution to journalArticle

Takamori, Shigeo ; Itonori, Saki ; Nakamura, Kyoko ; Suzuki, Minoru ; Suzuki, Akemi ; Inagaki, Fuyuhiko ; Shiota, Kunio ; Ogawa, Tomoya. / Ganglioside composition of GH3 cells : Enhancement of fucoganglioside expression by estradiol, epidermal growth factor and insulin. In: Biochimica et Biophysica Acta - Molecular Cell Research. 1998 ; Vol. 1401, No. 3. pp. 304-314.
@article{70e29ab415344168bc38343dc28e8730,
title = "Ganglioside composition of GH3 cells: Enhancement of fucoganglioside expression by estradiol, epidermal growth factor and insulin",
abstract = "The GH3 cell line, a bipotential cell line secreting both prolactin (PRL) and growth hormone (GH), is a useful model for investigating GH/PRL cell lineage differentiation and anterior pituitary adenoma formation. In this study, we investigated the ganglioside composition of GH3 cells and identified two fucogangliosides as the major gangliosides expressed by these cells. Analyses by DEAE-Sephadex A-25 and thin-layer chromatography (TLC) revealed that the GH3 cells contained two major gangliosides, designated FG1 and FG2, respectively. Their structures were identified by fast atom bombardment mass spectrometry and proton nuclear magnetic resonance spectrometry: FG1 is IV2Fucα,II3NeuAc-GgOse4Cer and FG2 is IV2Fucα,IV3Galα,II3NeuAc-GgOse4Cer. Expression of these fucogangliosides was enhanced by chronic treatment with 17β-estradiol (1 nM), epidermal growth factor (10 nM) and insulin (300 nM), which induced differentiation of GH3 cells to normal PRL-secreting cells. Interestingly, immunocytochemistry and flow cytometry revealed that the increased expression of these gangliosides reflected a quantitative change inside the cells but not on the cell surface. These results suggest that the intracellular distribution of fucogangliosides is closely related to the differentiation of GH3 cells.",
keywords = "Differentiation, Ganglioside, GH cell, Prolactin",
author = "Shigeo Takamori and Saki Itonori and Kyoko Nakamura and Minoru Suzuki and Akemi Suzuki and Fuyuhiko Inagaki and Kunio Shiota and Tomoya Ogawa",
year = "1998",
month = "3",
day = "5",
doi = "10.1016/S0167-4889(97)00139-0",
language = "English",
volume = "1401",
pages = "304--314",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Ganglioside composition of GH3 cells

T2 - Enhancement of fucoganglioside expression by estradiol, epidermal growth factor and insulin

AU - Takamori, Shigeo

AU - Itonori, Saki

AU - Nakamura, Kyoko

AU - Suzuki, Minoru

AU - Suzuki, Akemi

AU - Inagaki, Fuyuhiko

AU - Shiota, Kunio

AU - Ogawa, Tomoya

PY - 1998/3/5

Y1 - 1998/3/5

N2 - The GH3 cell line, a bipotential cell line secreting both prolactin (PRL) and growth hormone (GH), is a useful model for investigating GH/PRL cell lineage differentiation and anterior pituitary adenoma formation. In this study, we investigated the ganglioside composition of GH3 cells and identified two fucogangliosides as the major gangliosides expressed by these cells. Analyses by DEAE-Sephadex A-25 and thin-layer chromatography (TLC) revealed that the GH3 cells contained two major gangliosides, designated FG1 and FG2, respectively. Their structures were identified by fast atom bombardment mass spectrometry and proton nuclear magnetic resonance spectrometry: FG1 is IV2Fucα,II3NeuAc-GgOse4Cer and FG2 is IV2Fucα,IV3Galα,II3NeuAc-GgOse4Cer. Expression of these fucogangliosides was enhanced by chronic treatment with 17β-estradiol (1 nM), epidermal growth factor (10 nM) and insulin (300 nM), which induced differentiation of GH3 cells to normal PRL-secreting cells. Interestingly, immunocytochemistry and flow cytometry revealed that the increased expression of these gangliosides reflected a quantitative change inside the cells but not on the cell surface. These results suggest that the intracellular distribution of fucogangliosides is closely related to the differentiation of GH3 cells.

AB - The GH3 cell line, a bipotential cell line secreting both prolactin (PRL) and growth hormone (GH), is a useful model for investigating GH/PRL cell lineage differentiation and anterior pituitary adenoma formation. In this study, we investigated the ganglioside composition of GH3 cells and identified two fucogangliosides as the major gangliosides expressed by these cells. Analyses by DEAE-Sephadex A-25 and thin-layer chromatography (TLC) revealed that the GH3 cells contained two major gangliosides, designated FG1 and FG2, respectively. Their structures were identified by fast atom bombardment mass spectrometry and proton nuclear magnetic resonance spectrometry: FG1 is IV2Fucα,II3NeuAc-GgOse4Cer and FG2 is IV2Fucα,IV3Galα,II3NeuAc-GgOse4Cer. Expression of these fucogangliosides was enhanced by chronic treatment with 17β-estradiol (1 nM), epidermal growth factor (10 nM) and insulin (300 nM), which induced differentiation of GH3 cells to normal PRL-secreting cells. Interestingly, immunocytochemistry and flow cytometry revealed that the increased expression of these gangliosides reflected a quantitative change inside the cells but not on the cell surface. These results suggest that the intracellular distribution of fucogangliosides is closely related to the differentiation of GH3 cells.

KW - Differentiation

KW - Ganglioside

KW - GH cell

KW - Prolactin

UR - http://www.scopus.com/inward/record.url?scp=0032485091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032485091&partnerID=8YFLogxK

U2 - 10.1016/S0167-4889(97)00139-0

DO - 10.1016/S0167-4889(97)00139-0

M3 - Article

C2 - 9540820

AN - SCOPUS:0032485091

VL - 1401

SP - 304

EP - 314

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 3

ER -