Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1

Takamitsu Shirai, Saki Itonori, Tadashi Tai, Michael J. Soares, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Rcho-1 cell line, originally established from a rat choriocarcinoma, shows differentiation into placental trophoblastic giant cell-like cells and has been used to study the mechanism of placental function control. In the present study, we analysed the ganglioside composition of Rcho-1 cells by HPTLC orcinol/H2SO4, TLC/immunostaining and immunohistochemistry. Rcho-1 cells expressed GM3 and GD3 as the major gangliosides and CTH as major neutral glycolipid when they were cultured in growth medium (20% FCS) or transplanted beneath the kidney capsule. The expression of these gangliosides was strong in the undifferentiated small cells, whereas the completely differentiated giant cells showed poor staining with antibodies against the gangliosides. Under culture conditions to induce cell differentiation using horse serum (1-20% HS), the expression of GD3 was suppressed and re-expressed when the medium was changed to growth medium, suggesting that a change of ganglioside components may trigger and define the direction of terminal differentiation. Thus the composition of glycolipids is conserved in Rcho-1 cells and is similar to that of the rat placenta, where GM3 is dominant in mid-pregnancy and decreased in late pregnancy, whereas GD3 is low in mid- pregnancy and increased in late pregnancy.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalGlycoconjugate Journal
Volume13
Issue number3
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Choriocarcinoma
Gangliosides
Rats
Cells
Cell Line
Pregnancy
Chemical analysis
Glycolipids
Giant Cells
Growth
Placenta
Horses
Capsules
Cell Differentiation
Immunohistochemistry
Staining and Labeling
Kidney
Antibodies
Serum

Keywords

  • ganglioside
  • glycosphingolipid
  • rat choriocarcinoma cell line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Shirai, T., Itonori, S., Tai, T., Soares, M. J., Shiota, K., & Ogawa, T. (1996). Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1. Glycoconjugate Journal, 13(3), 415-421. https://doi.org/10.1007/BF00731474

Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1. / Shirai, Takamitsu; Itonori, Saki; Tai, Tadashi; Soares, Michael J.; Shiota, Kunio; Ogawa, Tomoya.

In: Glycoconjugate Journal, Vol. 13, No. 3, 1996, p. 415-421.

Research output: Contribution to journalArticle

Shirai, T, Itonori, S, Tai, T, Soares, MJ, Shiota, K & Ogawa, T 1996, 'Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1', Glycoconjugate Journal, vol. 13, no. 3, pp. 415-421. https://doi.org/10.1007/BF00731474
Shirai, Takamitsu ; Itonori, Saki ; Tai, Tadashi ; Soares, Michael J. ; Shiota, Kunio ; Ogawa, Tomoya. / Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1. In: Glycoconjugate Journal. 1996 ; Vol. 13, No. 3. pp. 415-421.
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