GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler

Motoki Takaku, Sara A. Grimm, Takashi Shimbo, Lalith Perera, Roberta Menafra, Hendrik G. Stunnenberg, Trevor K. Archer, Shinichi Machida, Hitoshi Kurumizaka, Paul A. Wade

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Background: Transcription factor-dependent cellular reprogramming is integral to normal development and is central to production of induced pluripotent stem cells. This process typically requires pioneer transcription factors (TFs) to induce de novo formation of enhancers at previously closed chromatin. Mechanistic information on this process is currently sparse. Results: Here we explore the mechanistic basis by which GATA3 functions as a pioneer TF in a cellular reprogramming event relevant to breast cancer, the mesenchymal to epithelial transition (MET). In some instances, GATA3 binds previously inaccessible chromatin, characterized by stable, positioned nucleosomes where it induces nucleosome eviction, alters local histone modifications, and remodels local chromatin architecture. At other loci, GATA3 binding induces nucleosome sliding without concomitant generation of accessible chromatin. Deletion of the transactivation domain retains the chromatin binding ability of GATA3 but cripples chromatin reprogramming ability, resulting in failure to induce MET. Conclusions: These data provide mechanistic insights into GATA3-mediated chromatin reprogramming during MET, and suggest unexpected complexity to TF pioneering. Successful reprogramming requires stable binding to a nucleosomal site; activation domain-dependent recruitment of co-factors including BRG1, the ATPase subunit of the SWI/SNF chromatin remodeling complex; and appropriate genomic context. The resulting model provides a new conceptual framework for de novo enhancer establishment by a pioneer TF.

    Original languageEnglish
    Article number36
    JournalGenome Biology
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 2016 Feb 27

    Fingerprint

    conceptual framework
    Chromatin
    sliding
    chromatin
    cancer
    genomics
    stem
    Transcription Factors
    Epithelial-Mesenchymal Transition
    Nucleosomes
    transcription factors
    nucleosomes
    Histone Code
    Induced Pluripotent Stem Cells
    Chromatin Assembly and Disassembly
    Cellular Reprogramming
    binding capacity
    transcriptional activation
    Transcriptional Activation
    Adenosine Triphosphatases

    Keywords

    • Breast cancer
    • Chromatin remodeling
    • Enhancer establishment
    • GATA3
    • Mesenchymal-to-epithelial transition
    • Pioneer factor

    ASJC Scopus subject areas

    • Cell Biology
    • Ecology, Evolution, Behavior and Systematics
    • Genetics

    Cite this

    Takaku, M., Grimm, S. A., Shimbo, T., Perera, L., Menafra, R., Stunnenberg, H. G., ... Wade, P. A. (2016). GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler. Genome Biology, 17(1), [36]. https://doi.org/10.1186/s13059-016-0897-0

    GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler. / Takaku, Motoki; Grimm, Sara A.; Shimbo, Takashi; Perera, Lalith; Menafra, Roberta; Stunnenberg, Hendrik G.; Archer, Trevor K.; Machida, Shinichi; Kurumizaka, Hitoshi; Wade, Paul A.

    In: Genome Biology, Vol. 17, No. 1, 36, 27.02.2016.

    Research output: Contribution to journalArticle

    Takaku, M, Grimm, SA, Shimbo, T, Perera, L, Menafra, R, Stunnenberg, HG, Archer, TK, Machida, S, Kurumizaka, H & Wade, PA 2016, 'GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler', Genome Biology, vol. 17, no. 1, 36. https://doi.org/10.1186/s13059-016-0897-0
    Takaku, Motoki ; Grimm, Sara A. ; Shimbo, Takashi ; Perera, Lalith ; Menafra, Roberta ; Stunnenberg, Hendrik G. ; Archer, Trevor K. ; Machida, Shinichi ; Kurumizaka, Hitoshi ; Wade, Paul A. / GATA3-dependent cellular reprogramming requires activation-domain dependent recruitment of a chromatin remodeler. In: Genome Biology. 2016 ; Vol. 17, No. 1.
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