Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo

R. N. D'Souza, A. Cavender, G. Sunavala, J. Alvarez, Toshio Ohshima, A. B. Kulkarni, M. Macdougall

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

Although the precise mechanisms of the conversion of predentin to dentin are not well understood, several lines of evidence implicate the noncollagenous proteins (NCPs) as important regulators of dentin biomineralization. Here we compared the in vivo temporospatial expression patterns of two dentin NCP genes, dentin matrix protein 1 (Dmp1), and dentin sialophosphoprotein (DSPP) in developing molars. Reverse transcription- polymerase chain reaction was performed on embryonic day 13 to 1-day-old first molars using Dmp1- and DSPP-specific primer sets. Dmp1 transcripts appeared at the late bud stage, while DSPP mRNA was seen at the cap stage. Expression of both genes was sustained throughout odontogenesis. In situ hybridization analysis revealed interesting differences in the expression patterns of these genes. While Dmp1 and DSPP showed coexpression in young odontoblasts before the start of mineralization, the expression of these genes was notably distinct at later stages. Dmp1 expression decreased in secretory odontoblasts after the appearance of mineral, while high levels of DSPP were sustained in odontoblasts. In early secretory ameloblasts, DSPP expression was transient and down-regulated with the appearance of dentin matrix. Interestingly, Dmp1 expression became evident in ameloblasts during the maturative phase of amelogenesis. In contrast to Dspp expression that was tooth-specific, Dmp1 was expressed by osteoblasts throughout ossification in the skeleton. Probes directed to the 'DSP' and 'DPP' regions of the DSPP gene showed identical patterns of mRNA expression. These data show that the developmental expression patterns of Dmp1 and DSPP are distinct, implying that these molecules serve different biological functions in vivo.

Original languageEnglish
Pages (from-to)2040-2049
Number of pages10
JournalJournal of Bone and Mineral Research
Volume12
Issue number12
Publication statusPublished - 1997 Dec
Externally publishedYes

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Dentin
Gene Expression
Proteins
Odontoblasts
Ameloblasts
dentin sialophosphoprotein
Amelogenesis
Odontogenesis
Messenger RNA
Osteoblasts
Osteogenesis
Skeleton
Reverse Transcription
In Situ Hybridization
Minerals
Tooth

ASJC Scopus subject areas

  • Surgery

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Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. / D'Souza, R. N.; Cavender, A.; Sunavala, G.; Alvarez, J.; Ohshima, Toshio; Kulkarni, A. B.; Macdougall, M.

In: Journal of Bone and Mineral Research, Vol. 12, No. 12, 12.1997, p. 2040-2049.

Research output: Contribution to journalArticle

D'Souza, R. N. ; Cavender, A. ; Sunavala, G. ; Alvarez, J. ; Ohshima, Toshio ; Kulkarni, A. B. ; Macdougall, M. / Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. In: Journal of Bone and Mineral Research. 1997 ; Vol. 12, No. 12. pp. 2040-2049.
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