Gene structure and transcriptional regulation of human Gal β1,4(3) GlcNac α2,3-sialyltransferase VI (hST3Gal VI) gene in prostate cancer cell line

Akiyoshi Taniguchi, Reiko Kaneta, Kayoko Morishita, Kojiro Matsumoto

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We describe transcriptional regulation of the human Gal β1,4(3) GlcNAc α2,3-sialyltransferase VI (hST3Gal VI) gene. The 5′-RACE results indicated that two mRNA forms differ only in the 5′-untranslated region (types 1 and 2). The genomic structure shows that the transcriptional regulation of type 1 and type 2 mRNA depends on the P1 and P2 promoters, respectively. Northern blots of RNA derived from various human tissues showed that the expression level of type 2 mRNA is higher than that of type 1 in the prostate. To elucidate the molecular basis of hST3Gal VI gene expression, we isolated and functionally characterized the genomic region containing the P1 and P2 promoters of hST3Gal VI. The activity of the P2 promoter is much higher than that of the P1 promoter in the prostate adenocarcinoma cell line PC-3. The results suggested that the hST3Gal VI gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of transcription.

Original languageEnglish
Pages (from-to)1148-1156
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number5
DOIs
Publication statusPublished - 2001 Oct 12
Externally publishedYes

Keywords

  • Dual promoter
  • Gene regulation
  • Luciferase assay
  • Multiple mRNA isoforms
  • Sialic acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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