Generation and characterization of a monoclonal antibody recognizing a petal brain enriched O-linked sialoglycoprotein, FOG100

Takuya Imamura, Naka Hattori, Kunio Shiota, Tomoya Ogawa

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1 Citation (Scopus)

Abstract

With the aim of identifying molecules that are expressed specifically in the brain during neurogenesis, we tried to generate monoclonal antibodies which recognize molecules showing unique temporal expression patterns and molecular characteristics. We used a homogenate of the rat fetal forebrain (day 12 of fetal life, E12) as an immunogen, and antibodies which reacted with this preparation were screened by immunoblotting. One of the antibodies, Mab3C8, recognized a 100-kDa antigen that is enriched in fetal brain. This 100-kDa antigen was constantly expressed during fetal life (from E12 to E20) and became scarcely detectable two days after birth. The antigen was detected in the insoluble fraction of fetal brain and its isoelectric point ranged from 6 to 7, suggesting that it was a membrane-coupled glycoprotein. Analysis by glycosidase treatment and lectin blotting suggested that it was an O-linked glycoprotein with an α2,6 sialyl linkage. Thus, a molecule unique to the fetal brain, an O-linked sialoglycoprotein with a molecular mass of 100 kDa (FOG100), was found by generating an antibody.

Original languageEnglish
Pages (from-to)534-539
Number of pages6
JournalJournal of Biochemistry
Volume124
Issue number3
Publication statusPublished - 1998 Sep
Externally publishedYes

Keywords

  • Fetal brain
  • FOG100
  • Monoclonal antibody
  • O-glycosylation
  • Sialoglycoprotein

ASJC Scopus subject areas

  • Biochemistry

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