Generation and characterization of mice lacking gastrin-releasing peptide receptor

Etsuko Wada, Kei Watase, Kazuyuki Yamada, Hiroo Ogura, Mariko Yamano, Yuji Inomata, Junichi Eguchi, Kazutoshi Yamamoto, Mary E. Sunday, Hiroshi Maeno, Katsuhiko Mikoshiba, Hiroko Ohki-Hamazaki, Keiji Wada

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    Gastrin-releasing peptide (GRP) is a mammalian bombesin-like peptide which is widely distributed in the central nervous system as well as in the gastrointestinal tract. GRP binds to its high affinity receptor (GRPR) to elicit a wide spectrum of biological effects on behavior, digestion, and metabolism. To define the in vivo function of GRPR, we generated GRPR null mutant mice by gene targeting. The intracerebroventricular administration of GRP caused hypothermia in wild-type mice, but not in mutant mice. The GRPR deficient mice showed significantly increased locomotor activity during the dark period, and social responses scored by sniffing, mounting, and approaching behaviors against an intruder. Aggressive scores such as fighting and biting were not altered in the mutant mice. These phenotypes were observed in mice generated from two independent ES cell clones and back-crossed to a C57BL/6J background. The GRPR deficient mice should be useful for studying the bombesin system in vivo.

    Original languageEnglish
    Pages (from-to)28-33
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume239
    Issue number1
    DOIs
    Publication statusPublished - 1997 Oct 9

    Fingerprint

    Bombesin Receptors
    Gastrin-Releasing Peptide
    Bombesin
    Clone cells
    Hypothermia
    Neurology
    Mountings
    Metabolism
    Genes
    Peptides
    Choice Behavior
    Gene Targeting
    Locomotion
    Gastrointestinal Tract
    Digestion
    Central Nervous System
    Clone Cells
    Phenotype

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Generation and characterization of mice lacking gastrin-releasing peptide receptor. / Wada, Etsuko; Watase, Kei; Yamada, Kazuyuki; Ogura, Hiroo; Yamano, Mariko; Inomata, Yuji; Eguchi, Junichi; Yamamoto, Kazutoshi; Sunday, Mary E.; Maeno, Hiroshi; Mikoshiba, Katsuhiko; Ohki-Hamazaki, Hiroko; Wada, Keiji.

    In: Biochemical and Biophysical Research Communications, Vol. 239, No. 1, 09.10.1997, p. 28-33.

    Research output: Contribution to journalArticle

    Wada, E, Watase, K, Yamada, K, Ogura, H, Yamano, M, Inomata, Y, Eguchi, J, Yamamoto, K, Sunday, ME, Maeno, H, Mikoshiba, K, Ohki-Hamazaki, H & Wada, K 1997, 'Generation and characterization of mice lacking gastrin-releasing peptide receptor', Biochemical and Biophysical Research Communications, vol. 239, no. 1, pp. 28-33. https://doi.org/10.1006/bbrc.1997.7418
    Wada, Etsuko ; Watase, Kei ; Yamada, Kazuyuki ; Ogura, Hiroo ; Yamano, Mariko ; Inomata, Yuji ; Eguchi, Junichi ; Yamamoto, Kazutoshi ; Sunday, Mary E. ; Maeno, Hiroshi ; Mikoshiba, Katsuhiko ; Ohki-Hamazaki, Hiroko ; Wada, Keiji. / Generation and characterization of mice lacking gastrin-releasing peptide receptor. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 239, No. 1. pp. 28-33.
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