Generation of phenoxy radicals by methemoglobin-hydrogen peroxide studied by electron paramagnetic resonance

Takeshi Shiga, Kazuhiko Imaizumi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The free radical intermediates of phenol derivatives, produced by the methemoglobin-hydrogen peroxide system at pH 5 and 7, are detected by electron paramagnetic resonance equipped with a continuous-flow apparatus. All the radicals from phenols are the phenoxy radicals, as identified by analyzing the observed hyperfine structures of the spectra with the aid of SCF-LCAO MO calculations. Comparing with the reaction of Fenton's reagent, it is concluded that free OH radical, even if it exists, is not liberated into the solution in the methemoglobin-hydrogen peroxide system.

Original languageEnglish
Pages (from-to)540-547
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume154
Issue number2
DOIs
Publication statusPublished - 1973
Externally publishedYes

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Methemoglobin
Electron Spin Resonance Spectroscopy
Hydrogen Peroxide
Free Radicals
Paramagnetic resonance
Phenols
Phenol
Free radicals
Derivatives
phenoxy radical
Fenton's reagent

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Generation of phenoxy radicals by methemoglobin-hydrogen peroxide studied by electron paramagnetic resonance. / Shiga, Takeshi; Imaizumi, Kazuhiko.

In: Archives of Biochemistry and Biophysics, Vol. 154, No. 2, 1973, p. 540-547.

Research output: Contribution to journalArticle

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