Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2

Masako Tanaka, Masayuki Shiota, Masaru Koyama, Jun Nakayama, Masakazu Yashiro, Kentaro Semba, Nobuhito Goda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume39
Issue number1
DOIs
Publication statusPublished - 2020 Feb

Keywords

  • SDF-2
  • mAbs
  • the rat medial iliac lymph method

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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