TY - JOUR
T1 - Generation of Rat Monoclonal Antibodies Specific for Human Stromal Cell-Derived Factor-2
AU - Tanaka, Masako
AU - Shiota, Masayuki
AU - Koyama, Masaru
AU - Nakayama, Jun
AU - Yashiro, Masakazu
AU - Semba, Kentaro
AU - Goda, Nobuhito
N1 - Funding Information:
This work was partly supported by JSPS KAKENHI Grant, number 17K15757, and the Uehara Memorial Foundation.
Publisher Copyright:
© Masako Tanaka et al. 2020; Published by Mary Ann Liebert, Inc. 2020.
PY - 2020/2
Y1 - 2020/2
N2 - Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells.
AB - Stromal cell-derived factor-2 (SDF-2) is reportedly involved in multiple endoplasmic reticulum (ER) functions, including the misfolded protein catabolic process, protein glycosylation, and ER protein quality control. However, the precise molecular and cellular functions of SDF-2 remain unknown. Previously, we discovered that SDF-2 mediates acquired resistance to oxaliplatin in human gastric cancer cells. In this study, we have generated SDF-2-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method, as a tool to explore novel mechanisms of oxaliplatin resistance. The antibodies detected endogenous human SDF-2 in immunoblotting analyses. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human SDF-2. Thus, these mAbs will be available to elucidate molecular and cellular functions of SDF-2 in cancer cells.
KW - SDF-2
KW - mAbs
KW - the rat medial iliac lymph method
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U2 - 10.1089/mab.2019.0043
DO - 10.1089/mab.2019.0043
M3 - Article
C2 - 31916900
AN - SCOPUS:85079789471
SN - 2167-9436
VL - 39
SP - 23
EP - 26
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -