Genetic analysis of biomagnetic crystal formation

Tadashi Matsunaga, N. Tsujimura, S. Kamiya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Magnetospirillum sp. AMB-1 is a freshwater magnetic bacterium which synthesizes intracellular particles of magnetite (Fe3O4). A genomic DNA fragment required for synthesis of magnetic particles was previously isolated from a non-magnetic transposon Tn5 mutant. We have determined the complete nucleotide sequence of this fragment. The 2975 bp region contains two putative open reading frames (ORFs). One ORF, designated magA, encodes a polypeptide which is homologous to the cation efflux proteins, the Escherichia coli potassium ion translocating protein, KefC, and the putative Na+/H+-antiporter, NapA, from Enterococcus hirae. Intracellular localization of the MagA protein was studied using magA - luc fusion proteins. The lue gene was cloned downstream of the magA hydrophilic C-terminal domain. The fusion protein was also detected on the surface of the lipid bilayer covering the magnetic particles. These results suggest that MagA is a membrane-bound protein. Vesicles which contained MagA protein exhibited iron accumulation ability. We consider that the MagA protein is an iron transporter involved in the synthesis of magnetic particles in AMB-1.

Original languageEnglish
JournalJournal De Physique. IV : JP
Volume7
Issue number1
Publication statusPublished - 1997 Mar
Externally publishedYes

Fingerprint

proteins
Proteins
Crystals
crystals
Fusion reactions
Iron
Ferrosoferric Oxide
fusion
fragments
Sodium-Hydrogen Antiporter
Lipid bilayers
efflux
Escherichia coli Proteins
iron
transporter
Polypeptides
polypeptides
nucleotides
Magnetite
synthesis

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Matsunaga, T., Tsujimura, N., & Kamiya, S. (1997). Genetic analysis of biomagnetic crystal formation. Journal De Physique. IV : JP, 7(1).

Genetic analysis of biomagnetic crystal formation. / Matsunaga, Tadashi; Tsujimura, N.; Kamiya, S.

In: Journal De Physique. IV : JP, Vol. 7, No. 1, 03.1997.

Research output: Contribution to journalArticle

Matsunaga, T, Tsujimura, N & Kamiya, S 1997, 'Genetic analysis of biomagnetic crystal formation', Journal De Physique. IV : JP, vol. 7, no. 1.
Matsunaga T, Tsujimura N, Kamiya S. Genetic analysis of biomagnetic crystal formation. Journal De Physique. IV : JP. 1997 Mar;7(1).
Matsunaga, Tadashi ; Tsujimura, N. ; Kamiya, S. / Genetic analysis of biomagnetic crystal formation. In: Journal De Physique. IV : JP. 1997 ; Vol. 7, No. 1.
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