Genetic analysis of magnetic bacteria

Research output: Contribution to journalArticle

Abstract

Magnetospirillum sp. AMB-1 is a freshwater magnetic bacterium which synthesizes intracellular particles of magnetite (Fe3O4). Agenomic DNA fragment required for synthesis of magnetic particles was previously isolated from a nonmagnetic mutant. We have determined the complete nucleotide sequence of this fragment. The 2975 bp region contains two putative open reading frames. One ORF, designated magA, encodes a polypeptide which is homologous to the cation efflux proteins, the Escherichia coli potassium ion translocating protein, KefC. Inverted vesicles prepared from Escherichia coli cell membrane, in which the MagA protein was expressed, exhibited iron accumulation ability. We consider that the MagA protein is an iron transport protein involved in the synthesis of magnetic particles in AMB-1. Intracellular localization of the MagA protein was studied using magA-luc fusion proteins. The fusion protein was also detected on the surface of the lipid bilayer covering the magnetic particles. These results suggest that MagA is a membrane-bound protein.

Original languageEnglish
Pages (from-to)287-289
Number of pages3
JournalMaterials Science and Engineering C
Volume4
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

bacteria
Bacteria
proteins
Proteins
Escherichia coli
Escherichia
Fusion reactions
Iron
Ferrosoferric Oxide
Lipid bilayers
fusion
fragments
Escherichia coli Proteins
Cell membranes
efflux
iron
Polypeptides
Magnetite
Nucleotides
Cations

Keywords

  • Fusion protein
  • magA gene
  • Magnetic bacteria

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Genetic analysis of magnetic bacteria. / Matsunaga, T.

In: Materials Science and Engineering C, Vol. 4, No. 4, 1997, p. 287-289.

Research output: Contribution to journalArticle

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