Genetic engineering in marine cyanobacteria

Tadashi Matsunaga, Haruko Takeyama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Many species of microalgae producing useful materials have been isolated from marine environments. For their industrial application, widely applicable and stable gene expression is required. It is necessary to establish gene transfer methods as an essential first step in genetic manipulation. Although gene transfer techniques for cyanobacteria have been developed, only naturally transformable strains have been used. Here, we describe recent progress made in developing gene transfer methods for marine cyanobacteria. The following are covered: (1) transformation, (2) electroporation, (3) conjugation, (4) particle gun. A plasmid from the marine cyanobacterium, Synechococcus sp., whose copy number is dependent on salinity, was characterized. This plasmid is being used to develop a stable and controllable gene expression system.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalJournal of Applied Phycology
Volume7
Issue number1
DOIs
Publication statusPublished - 1995 Feb 1
Externally publishedYes

Keywords

  • Synechococcus gene transfer
  • conjugation
  • electroporation
  • marine cyanobacteria
  • particle gun
  • transformation

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Fingerprint Dive into the research topics of 'Genetic engineering in marine cyanobacteria'. Together they form a unique fingerprint.

  • Cite this