Genetic engineering in marine cyanobacteria

Tadashi Matsunaga, Haruko Takeyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Many species of microalgae producing useful materials have been isolated from marine environments. For their industrial application, widely applicable and stable gene expression is required. It is necessary to establish gene transfer methods as an essential first step in genetic manipulation. Although gene transfer techniques for cyanobacteria have been developed, only naturally transformable strains have been used. Here, we describe recent progress made in developing gene transfer methods for marine cyanobacteria. The following are covered: (1) transformation, (2) electroporation, (3) conjugation, (4) particle gun. A plasmid from the marine cyanobacterium, Synechococcus sp., whose copy number is dependent on salinity, was characterized. This plasmid is being used to develop a stable and controllable gene expression system.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalJournal of Applied Phycology
Volume7
Issue number1
DOIs
Publication statusPublished - 1995 Feb
Externally publishedYes

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Keywords

  • conjugation
  • electroporation
  • marine cyanobacteria
  • particle gun
  • Synechococcus gene transfer
  • transformation

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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