@article{ff943395c08e4f0da76e739604c77131,
title = "Genetic inhibition of CRMP2 phosphorylation delays Wallerian degeneration after optic nerve injury",
abstract = "Axonal degeneration occurs in patients with various neurological diseases and traumatic nerve injuries, and Wallerian degeneration is a phenomenon in the prototypical axonal degradation that is observed after injury. Collapsin response mediator protein 2 (CRMP2)is phosphorylated by glycogen synthase kinase 3β (GSK3β), and it is involved in Wallerian degeneration after optic nerve injury. We previously developed a CRMP2 knock-in (CRMP2 KI)mouse line, in which CRMP2 phosphorylation by GSK3β is inhibited; however, Wallerian degeneration in CRMP2 KI mice has not yet been examined. In this study, we examined whether Wallerian degeneration of the optic nerve is suppressed in CRMP2 KI mice. Using one eye removal model, we compared Wallerian degeneration of the optic nerve based on histological and biochemical analyses. Our experimental results indicated that the genetic inhibition of CRMP2 phosphorylation delays Wallerian degeneration after optic nerve injury.",
keywords = "Genetic, Mouse, Neurodegeneration, Optic nerve, Phosphorylation",
author = "Yuki Kinoshita and Syunsuke Kondo and Kazuya Takahashi and Jun Nagai and Shuji Wakatsuki and Toshiyuki Araki and Yoshio Goshima and Toshio Ohshima",
note = "Funding Information: We thank Dr. Koriyama in Shiga University of Medical Science for the advice for procedure of optic nerve injury. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Culture, Sports, Science and Technology (no. 26430043 to T. O and no. 17082006 to Y.G.), and Core Research for Evolutional Science and Technology of Japan Science and Technology Agency (Y.G.)and Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program in the Project for Developing Innovation System from the MEXT (no. 42890001 to YG). Funding Information: We thank Dr. Koriyama in Shiga University of Medical Science for the advice for procedure of optic nerve injury. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas from The Ministry of Education, Culture, Sports, Science and Technology (no. 26430043 to T. O and no. 17082006 to Y.G.), and Core Research for Evolutional Science and Technology of Japan Science and Technology Agency (Y.G.) and Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program in the Project for Developing Innovation System from the MEXT (no. 42890001 to YG). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = jul,
day = "5",
doi = "10.1016/j.bbrc.2019.05.060",
language = "English",
volume = "514",
pages = "1037--1039",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",
}