Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae

Satoshi Yoshida, Yoshikazu Ohya, Akihiko Nakano, Yasuhiro Anraku

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Loss of yeast protein kinase C function results in three distinct phenotypes: staurosporine sensitivity, cell lysis and blockage of cell cycle progression at the G2/M boundary. Genetic analysis of the PKC1/STT1 protein kinase C gene and its interactions with STT4, encoding an upstream phosphatidylinositol 4-kinase, and BCK1, encoding a downstream protein kinase, reveal that they form part of a single pathway. However, the BCK1-20 mutation (a gain-of-function mutation of BCK1) or overexpression of PKC1 cannot suppress all of the phenotypes caused by the loss of STT4 function, strongly suggesting the existence of a branch point between STT4 and PKC1. We also describe the MSS4 gene, a multicopy suppressor of the temperature-sensitive stt4-1 mutation. MSS4 is predicted to encode a hydrophilic protein of 779 amino acid residues and is essential for cell growth. Based on genetic and biochemical data, we suggest that MSS4 acts downstream of STT4, but in a pathway that does not involve PKC1.

Original languageEnglish
Pages (from-to)631-640
Number of pages10
JournalMGG Molecular & General Genetics
Volume242
Issue number6
DOIs
Publication statusPublished - 1994 Mar 1
Externally publishedYes

Fingerprint

Saccharomyces cerevisiae
Protein Kinase C
Mutation
Genes
Phenotype
1-Phosphatidylinositol 4-Kinase
Fungal Proteins
Staurosporine
Protein Kinases
Molecular Biology
Cell Cycle
Amino Acids
Temperature
Growth
Proteins

Keywords

  • Cell cycle
  • Cell lysis
  • Phosphatidylinositol 4-kinase
  • Protein kinase C
  • Staurosporine

ASJC Scopus subject areas

  • Genetics

Cite this

Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae. / Yoshida, Satoshi; Ohya, Yoshikazu; Nakano, Akihiko; Anraku, Yasuhiro.

In: MGG Molecular & General Genetics, Vol. 242, No. 6, 01.03.1994, p. 631-640.

Research output: Contribution to journalArticle

Yoshida, Satoshi ; Ohya, Yoshikazu ; Nakano, Akihiko ; Anraku, Yasuhiro. / Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae. In: MGG Molecular & General Genetics. 1994 ; Vol. 242, No. 6. pp. 631-640.
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