Genome mining approach for the discovery of novel cytochrome P450 biocatalysts

Toshiki Furuya, Kuniki Kino

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Cytochrome P450 enzymes (P450s) are able to regioselectively and stereoselectively introduce oxygen into organic compounds under mild reaction conditions. These monooxygenases in particular easily catalyze the insertion of oxygen into less reactive carbon-hydrogen bonds. Hence, P450s are of considerable interest as oxidation biocatalysts. To date, although several P450s have been discovered through screening of microorganisms and have been further genetically engineered, the substrate range of these biocatalysts is still limited to fulfill the requirements for a large number of oxidation processes. On the other hand, the recent rapid expansion in the number of reported microbial genome sequences has revealed the presence of an unexpectedly vast number of P450 genes. This large pool of naturally evolved P450s has attracted much attention as a resource for new oxidation biocatalysts. In this review, we focus on aspects of the genome mining approach that are relevant for the discovery of novel P450 biocatalysts. This approach opens up possibilities for exploitation of the catalytic potential of P450s for the preparation of a large choice of oxidation biocatalysts with a variety of substrate specificities.

    Original languageEnglish
    Pages (from-to)991-1002
    Number of pages12
    JournalApplied Microbiology and Biotechnology
    Volume86
    Issue number4
    DOIs
    Publication statusPublished - 2010 Apr

    Fingerprint

    Cytochrome P-450 Enzyme System
    Genome
    Enzymes
    Microbial Genome
    Oxygen
    Substrate Specificity
    Mixed Function Oxygenases
    Hydrogen
    Carbon
    Genes

    Keywords

    • Biocatalyst
    • Cytochrome P450
    • Genome mining
    • Hydroxylation
    • Monooxygenase
    • Oxidation

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Cite this

    Genome mining approach for the discovery of novel cytochrome P450 biocatalysts. / Furuya, Toshiki; Kino, Kuniki.

    In: Applied Microbiology and Biotechnology, Vol. 86, No. 4, 04.2010, p. 991-1002.

    Research output: Contribution to journalArticle

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