Genome sequence of an M3 strain of streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution

Ichiro Nakagawa, Ken Kurokawa, Atsushi Yamashita, Masanobu Nakata, Yusuke Tomiyasu, Nobuo Okahashi, Shigetada Kawabata, Kiyoshi Yamazaki, Tadayoshi Shiba, Teruo Yasunaga, Hideo Hayashi, Masahira Hattori, Shigeyuki Hamada

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Group A streptococcus (GAS) is a gram-positive bacterial pathogen that causes various suppurative infections and nonsuppurative sequelae. Since the late 1980s, streptococcal toxic-shock like syndrome (STSS) and severe invasive GAS infections have been reported globally. Here we sequenced the genome of serotype M3 strain SSI-1, isolated from an STSS patient in Japan, and compared it with those of other GAS strains. The SSI-I genome is composed of 1,884,275 bp, and 1.7 Mb of the sequence is highly conserved relative to strain SF370 (serotype M1) and MGAS8232 (serotype M18), and almost completely conserved relative to strain MGAS315 (serotype M3). However, a large genomic rearrangement has been shown to occur across the replication axis between the homologous rrn-comX1 regions and between two prophage-coding regions across the replication axis. A total of 1 Mb of chromosomal DNA is inverted across the replication axis. Interestingly, the recombinations between the prophage regions are within the phage genes, and the genes encoding superantigens and mitogenic factors are interchanged between two prophages. This genomic rearrangement occurs in 65% of clinical isolates (64/94) collected after 1990, whereas it is found in only 25% of clinical isolates (7/28) collected before 1985. These observations indicate that streptococcal phages represent important plasticity regions in the GAS chromosome where recombination between homologous phage genes can occur and result not only in new phage derivatives, but also in large chromosomal rearrangements.

Original languageEnglish
Pages (from-to)1042-1055
Number of pages14
JournalGenome Research
Volume13
Issue number6 A
DOIs
Publication statusPublished - 2003 Jun 1
Externally publishedYes

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Streptococcus pyogenes
Streptococcus
Prophages
Bacteriophages
Genome
Septic Shock
Genetic Recombination
Streptococcus Phages
Genes
Chromosomes, Human, 1-3
Superantigens
Infection
Japan
DNA
Serogroup

ASJC Scopus subject areas

  • Genetics

Cite this

Genome sequence of an M3 strain of streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution. / Nakagawa, Ichiro; Kurokawa, Ken; Yamashita, Atsushi; Nakata, Masanobu; Tomiyasu, Yusuke; Okahashi, Nobuo; Kawabata, Shigetada; Yamazaki, Kiyoshi; Shiba, Tadayoshi; Yasunaga, Teruo; Hayashi, Hideo; Hattori, Masahira; Hamada, Shigeyuki.

In: Genome Research, Vol. 13, No. 6 A, 01.06.2003, p. 1042-1055.

Research output: Contribution to journalArticle

Nakagawa, I, Kurokawa, K, Yamashita, A, Nakata, M, Tomiyasu, Y, Okahashi, N, Kawabata, S, Yamazaki, K, Shiba, T, Yasunaga, T, Hayashi, H, Hattori, M & Hamada, S 2003, 'Genome sequence of an M3 strain of streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution', Genome Research, vol. 13, no. 6 A, pp. 1042-1055. https://doi.org/10.1101/gr.1096703
Nakagawa, Ichiro ; Kurokawa, Ken ; Yamashita, Atsushi ; Nakata, Masanobu ; Tomiyasu, Yusuke ; Okahashi, Nobuo ; Kawabata, Shigetada ; Yamazaki, Kiyoshi ; Shiba, Tadayoshi ; Yasunaga, Teruo ; Hayashi, Hideo ; Hattori, Masahira ; Hamada, Shigeyuki. / Genome sequence of an M3 strain of streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution. In: Genome Research. 2003 ; Vol. 13, No. 6 A. pp. 1042-1055.
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AU - Nakata, Masanobu

AU - Tomiyasu, Yusuke

AU - Okahashi, Nobuo

AU - Kawabata, Shigetada

AU - Yamazaki, Kiyoshi

AU - Shiba, Tadayoshi

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AU - Hayashi, Hideo

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AU - Hamada, Shigeyuki

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