Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS

Shuji Shigenobu, Hidemi Watanabe, Masahira Hattori, Yoshiyuki Sakaki, Hajime Ishikawa

Research output: Contribution to journalArticle

900 Citations (Scopus)

Abstract

Almost all aphid species (Homoptera, Insecta) have 60-80 huge cells called bacteriocytes, within which are round-shaped bacteria that are designated Buchnera. These bacteria are maternally transmitted to eggs and embryos through host generations, and the mutualism between the host and the bacteria is so obligate that neither can reproduce independently. Buchnera is a close relative of Escherichia coli, but it contains more than 100 genomic copies per cell, and its genome size is only a seventh of that of E. coli. Here we report the complete genome sequence of Buchnera sp. strain APS, which is composed of one 640,681-base-pair chromosome and two small plasmids. There are genes for the biosyntheses of amino acids essential for the hosts in the genome, but those for non-essential amino acids are missing, indicating complementarity and syntrophy between the host and the symbiont. In addition, Buchnera lacks genes for the biosynthesis of cell-surface components, including lipopolysaccharides and phospholipids, regulator genes and genes involved in defence of the cell. These results indicate that Buchnera is completely symbiotic and viable only in its limited niche, the bacteriocyte.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalNature
Volume407
Issue number6800
DOIs
Publication statusPublished - 2000 Sep
Externally publishedYes

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Buchnera
Aphids
Genome
Bacteria
Escherichia coli
Genes
Genome Size
Hemiptera
Essential Amino Acids
Symbiosis
Cellular Structures
Regulator Genes
Base Pairing
Eggs
Insects
Lipopolysaccharides
Phospholipids
Plasmids
Embryonic Structures
Chromosomes

ASJC Scopus subject areas

  • General

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Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS. / Shigenobu, Shuji; Watanabe, Hidemi; Hattori, Masahira; Sakaki, Yoshiyuki; Ishikawa, Hajime.

In: Nature, Vol. 407, No. 6800, 09.2000, p. 81-86.

Research output: Contribution to journalArticle

Shigenobu, S, Watanabe, H, Hattori, M, Sakaki, Y & Ishikawa, H 2000, 'Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS', Nature, vol. 407, no. 6800, pp. 81-86. https://doi.org/10.1038/35024074
Shigenobu, Shuji ; Watanabe, Hidemi ; Hattori, Masahira ; Sakaki, Yoshiyuki ; Ishikawa, Hajime. / Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS. In: Nature. 2000 ; Vol. 407, No. 6800. pp. 81-86.
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