Genome sequence of Vibrio parahaemolyticus

A pathogenic mechanism distinct from that of V cholerae

Kozo Makino, Kenshiro Oshima, Ken Kurokawa, Katsushi Yokoyama, Takayuki Uda, Kenichi Tagomori, Yoshio Iijima, Masatomo Najima, Masayuki Nakano, Atsushi Yamashita, Yoshino Kubota, Shigenobu Kimura, Teruo Yasunaga, Takeshi Honda, Hideo Shinagawa, Masahira Hattori, Tetsuya Iida

Research output: Contribution to journalArticle

614 Citations (Scopus)

Abstract

Background: Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. V parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. The organism is phylogenetically close to V cholerae, the causative agent of cholera. Methods: The whole genome sequence of a clinical V parahaemolyticus strain RIMD2210633 was established by shotgun sequencing. The coding sequences were identified by use of Gambler and Glimmer programs. Comparative analysis with the V cholerae genome was undertaken with MUMmer. Findings: The genome consisted of two circular chromosomes of 3 288 558 bp and 1 877 212 bp; it contained 4832 genes. Comparison of the V parahaemolyticus genome with that of V choleraeshowed many rearrangements within and between the two chromosomes. Genes for the type III secretion system (TTSS) were identified in the genome of V parahaemolyticus; V cholerae does not have these genes. Interpretation: The TTSS is a central virulence factor of diarrhoea-causing bacteria such as shigella, salmonella, and enteropathogenic Escherichia coli, which cause gastroenteritis by invading or intimately interacting with intestinal epithelial cells. Our results suggest that V parahaemolyticus and V cholerae use distinct mechanisms to establish infection. This finding explains clinical features of V parahaemolyticus infections, which commonly include inflammatory diarrhoea and in some cases systemic manifestations such as septicaemia, distinct from those of V cholerae infections, which are generally associated with non-inflammatory diarrhoea.

Original languageEnglish
Pages (from-to)743-749
Number of pages7
JournalLancet
Volume361
Issue number9359
DOIs
Publication statusPublished - 2003 Mar 1
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Cholera
Genome
Gastroenteritis
Diarrhea
Infection
Genes
Enteropathogenic Escherichia coli
Chromosomes, Human, Pair 3
Shigella
Firearms
Pandemics
Virulence Factors
Gram-Negative Bacteria
Salmonella
Sepsis
Chromosomes
Epithelial Cells
Bacteria
Food

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Makino, K., Oshima, K., Kurokawa, K., Yokoyama, K., Uda, T., Tagomori, K., ... Iida, T. (2003). Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V cholerae. Lancet, 361(9359), 743-749. https://doi.org/10.1016/S0140-6736(03)12659-1

Genome sequence of Vibrio parahaemolyticus : A pathogenic mechanism distinct from that of V cholerae. / Makino, Kozo; Oshima, Kenshiro; Kurokawa, Ken; Yokoyama, Katsushi; Uda, Takayuki; Tagomori, Kenichi; Iijima, Yoshio; Najima, Masatomo; Nakano, Masayuki; Yamashita, Atsushi; Kubota, Yoshino; Kimura, Shigenobu; Yasunaga, Teruo; Honda, Takeshi; Shinagawa, Hideo; Hattori, Masahira; Iida, Tetsuya.

In: Lancet, Vol. 361, No. 9359, 01.03.2003, p. 743-749.

Research output: Contribution to journalArticle

Makino, K, Oshima, K, Kurokawa, K, Yokoyama, K, Uda, T, Tagomori, K, Iijima, Y, Najima, M, Nakano, M, Yamashita, A, Kubota, Y, Kimura, S, Yasunaga, T, Honda, T, Shinagawa, H, Hattori, M & Iida, T 2003, 'Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V cholerae', Lancet, vol. 361, no. 9359, pp. 743-749. https://doi.org/10.1016/S0140-6736(03)12659-1
Makino K, Oshima K, Kurokawa K, Yokoyama K, Uda T, Tagomori K et al. Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V cholerae. Lancet. 2003 Mar 1;361(9359):743-749. https://doi.org/10.1016/S0140-6736(03)12659-1
Makino, Kozo ; Oshima, Kenshiro ; Kurokawa, Ken ; Yokoyama, Katsushi ; Uda, Takayuki ; Tagomori, Kenichi ; Iijima, Yoshio ; Najima, Masatomo ; Nakano, Masayuki ; Yamashita, Atsushi ; Kubota, Yoshino ; Kimura, Shigenobu ; Yasunaga, Teruo ; Honda, Takeshi ; Shinagawa, Hideo ; Hattori, Masahira ; Iida, Tetsuya. / Genome sequence of Vibrio parahaemolyticus : A pathogenic mechanism distinct from that of V cholerae. In: Lancet. 2003 ; Vol. 361, No. 9359. pp. 743-749.
@article{e65e31fd877341269f47f40fd1ea4724,
title = "Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V cholerae",
abstract = "Background: Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. V parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. The organism is phylogenetically close to V cholerae, the causative agent of cholera. Methods: The whole genome sequence of a clinical V parahaemolyticus strain RIMD2210633 was established by shotgun sequencing. The coding sequences were identified by use of Gambler and Glimmer programs. Comparative analysis with the V cholerae genome was undertaken with MUMmer. Findings: The genome consisted of two circular chromosomes of 3 288 558 bp and 1 877 212 bp; it contained 4832 genes. Comparison of the V parahaemolyticus genome with that of V choleraeshowed many rearrangements within and between the two chromosomes. Genes for the type III secretion system (TTSS) were identified in the genome of V parahaemolyticus; V cholerae does not have these genes. Interpretation: The TTSS is a central virulence factor of diarrhoea-causing bacteria such as shigella, salmonella, and enteropathogenic Escherichia coli, which cause gastroenteritis by invading or intimately interacting with intestinal epithelial cells. Our results suggest that V parahaemolyticus and V cholerae use distinct mechanisms to establish infection. This finding explains clinical features of V parahaemolyticus infections, which commonly include inflammatory diarrhoea and in some cases systemic manifestations such as septicaemia, distinct from those of V cholerae infections, which are generally associated with non-inflammatory diarrhoea.",
author = "Kozo Makino and Kenshiro Oshima and Ken Kurokawa and Katsushi Yokoyama and Takayuki Uda and Kenichi Tagomori and Yoshio Iijima and Masatomo Najima and Masayuki Nakano and Atsushi Yamashita and Yoshino Kubota and Shigenobu Kimura and Teruo Yasunaga and Takeshi Honda and Hideo Shinagawa and Masahira Hattori and Tetsuya Iida",
year = "2003",
month = "3",
day = "1",
doi = "10.1016/S0140-6736(03)12659-1",
language = "English",
volume = "361",
pages = "743--749",
journal = "The Lancet",
issn = "0140-6736",
publisher = "Elsevier Limited",
number = "9359",

}

TY - JOUR

T1 - Genome sequence of Vibrio parahaemolyticus

T2 - A pathogenic mechanism distinct from that of V cholerae

AU - Makino, Kozo

AU - Oshima, Kenshiro

AU - Kurokawa, Ken

AU - Yokoyama, Katsushi

AU - Uda, Takayuki

AU - Tagomori, Kenichi

AU - Iijima, Yoshio

AU - Najima, Masatomo

AU - Nakano, Masayuki

AU - Yamashita, Atsushi

AU - Kubota, Yoshino

AU - Kimura, Shigenobu

AU - Yasunaga, Teruo

AU - Honda, Takeshi

AU - Shinagawa, Hideo

AU - Hattori, Masahira

AU - Iida, Tetsuya

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Background: Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. V parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. The organism is phylogenetically close to V cholerae, the causative agent of cholera. Methods: The whole genome sequence of a clinical V parahaemolyticus strain RIMD2210633 was established by shotgun sequencing. The coding sequences were identified by use of Gambler and Glimmer programs. Comparative analysis with the V cholerae genome was undertaken with MUMmer. Findings: The genome consisted of two circular chromosomes of 3 288 558 bp and 1 877 212 bp; it contained 4832 genes. Comparison of the V parahaemolyticus genome with that of V choleraeshowed many rearrangements within and between the two chromosomes. Genes for the type III secretion system (TTSS) were identified in the genome of V parahaemolyticus; V cholerae does not have these genes. Interpretation: The TTSS is a central virulence factor of diarrhoea-causing bacteria such as shigella, salmonella, and enteropathogenic Escherichia coli, which cause gastroenteritis by invading or intimately interacting with intestinal epithelial cells. Our results suggest that V parahaemolyticus and V cholerae use distinct mechanisms to establish infection. This finding explains clinical features of V parahaemolyticus infections, which commonly include inflammatory diarrhoea and in some cases systemic manifestations such as septicaemia, distinct from those of V cholerae infections, which are generally associated with non-inflammatory diarrhoea.

AB - Background: Vibrio parahaemolyticus, a gram-negative marine bacterium, is a worldwide cause of food-borne gastroenteritis. V parahaemolyticus strains of a few specific serotypes, probably derived from a common clonal ancestor, have lately caused a pandemic of gastroenteritis. The organism is phylogenetically close to V cholerae, the causative agent of cholera. Methods: The whole genome sequence of a clinical V parahaemolyticus strain RIMD2210633 was established by shotgun sequencing. The coding sequences were identified by use of Gambler and Glimmer programs. Comparative analysis with the V cholerae genome was undertaken with MUMmer. Findings: The genome consisted of two circular chromosomes of 3 288 558 bp and 1 877 212 bp; it contained 4832 genes. Comparison of the V parahaemolyticus genome with that of V choleraeshowed many rearrangements within and between the two chromosomes. Genes for the type III secretion system (TTSS) were identified in the genome of V parahaemolyticus; V cholerae does not have these genes. Interpretation: The TTSS is a central virulence factor of diarrhoea-causing bacteria such as shigella, salmonella, and enteropathogenic Escherichia coli, which cause gastroenteritis by invading or intimately interacting with intestinal epithelial cells. Our results suggest that V parahaemolyticus and V cholerae use distinct mechanisms to establish infection. This finding explains clinical features of V parahaemolyticus infections, which commonly include inflammatory diarrhoea and in some cases systemic manifestations such as septicaemia, distinct from those of V cholerae infections, which are generally associated with non-inflammatory diarrhoea.

UR - http://www.scopus.com/inward/record.url?scp=0037333881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037333881&partnerID=8YFLogxK

U2 - 10.1016/S0140-6736(03)12659-1

DO - 10.1016/S0140-6736(03)12659-1

M3 - Article

VL - 361

SP - 743

EP - 749

JO - The Lancet

JF - The Lancet

SN - 0140-6736

IS - 9359

ER -