Genome-Wide Analysis of Acute Endurance Exercise-Induced Translational Regulation in Mouse Skeletal Muscle

Hiroaki Sako, Koichi Yada, Katsuhiko Suzuki

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Exercise dynamically changes skeletal muscle protein synthesis to respond and adapt to the external and internal stimuli. Many studies have focused on overall protein synthesis to understand how exercise regulates the muscular adaptation. However, despite the probability that each gene transcript may have its own unique translational characteristics and would be differentially regulated at translational level, little attention has been paid to how exercise affects translational regulation of individual genes at a genome-wide scale. Here, we conducted a genome-wide translational analysis using ribosome profiling to investigate the effect of a single bout of treadmill running (20 m/min for 60 min) on mouse gastrocnemius. Global translational profiles largely differed from those in transcription even at a basal resting condition as well as immediately after exercise. As for individual gene, Slc25a25 (Solute carrier family 25, member 25), localized in mitochondrial inner membrane and maintaining ATP homeostasis and endurance performance, showed significant up-regulation at translational level. However, multiple regression analysis suggests that Slc25a25 protein degradation may also have a role in mediating Slc25a25 protein abundance in the basal and early stages after acute endurance exercise.

    Original languageEnglish
    Article numbere0148311
    JournalPLoS One
    Volume11
    Issue number2
    DOIs
    Publication statusPublished - 2016 Feb 1

    Fingerprint

    Muscle
    skeletal muscle
    Skeletal Muscle
    Durability
    exercise
    Genes
    Genome
    Exercise
    genome
    mice
    Muscle Proteins
    Mitochondrial Membranes
    Ribosomes
    Running
    protein synthesis
    Proteolysis
    Proteins
    Homeostasis
    Up-Regulation
    Adenosine Triphosphate

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Genome-Wide Analysis of Acute Endurance Exercise-Induced Translational Regulation in Mouse Skeletal Muscle. / Sako, Hiroaki; Yada, Koichi; Suzuki, Katsuhiko.

    In: PLoS One, Vol. 11, No. 2, e0148311, 01.02.2016.

    Research output: Contribution to journalArticle

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