Genome-wide profiling of DNA methylation in human cancer cells

Katsumi Ogoshi, Shin ichi Hashimoto, Yoichiro Nakatani, Wei Qu, Kenshiro Oshima, Katsushi Tokunaga, Sumio Sugano, Masahira Hattori, Shinichi Morishita, Kouji Matsushima

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Global changes in DNA methylation correlate with altered gene expression and genomic instability in cancer. We have developed a methylation-specific digital sequencing (MSDS) method that can assess DNA methylation on a genomic scale. MSDS is a simple, low-cost method that combines the use of methylation-sensitive restriction enzymes with second generation sequencing technology. DNA methylation in two colon cancer cell lines, HT29 and HCT116, was measured using MSDS. When methylation levels were compared between the two cell lines, many differentially methylated regions (DMRs) were identified in CpG island shore regions (located within 2 kb of a CpG island), gene body regions and intergenic regions. The number of DMRs in the vicinity of gene transcription start sites correlated with the level of expression of TACC1, CLDN1, and PLEKHC1 (FERMT2) genes, which have been linked to carcinogenesis. The MSDS method has the potential to provide novel insight into the functional complexity of the human genome.

Original languageEnglish
Pages (from-to)280-287
Number of pages8
JournalGenomics
Volume98
Issue number4
DOIs
Publication statusPublished - 2011 Oct
Externally publishedYes

Fingerprint

DNA Methylation
Methylation
Genome
Neoplasms
CpG Islands
Genes
Cell Line
Body Regions
Intergenic DNA
Genomic Instability
Transcription Initiation Site
Human Genome
Colonic Neoplasms
Carcinogenesis
Technology
Gene Expression
Costs and Cost Analysis
Enzymes

Keywords

  • Colon cancer
  • Differentially methylated regions
  • DNA methylation
  • Second generation sequencing technology

ASJC Scopus subject areas

  • Genetics

Cite this

Ogoshi, K., Hashimoto, S. I., Nakatani, Y., Qu, W., Oshima, K., Tokunaga, K., ... Matsushima, K. (2011). Genome-wide profiling of DNA methylation in human cancer cells. Genomics, 98(4), 280-287. https://doi.org/10.1016/j.ygeno.2011.07.003

Genome-wide profiling of DNA methylation in human cancer cells. / Ogoshi, Katsumi; Hashimoto, Shin ichi; Nakatani, Yoichiro; Qu, Wei; Oshima, Kenshiro; Tokunaga, Katsushi; Sugano, Sumio; Hattori, Masahira; Morishita, Shinichi; Matsushima, Kouji.

In: Genomics, Vol. 98, No. 4, 10.2011, p. 280-287.

Research output: Contribution to journalArticle

Ogoshi, K, Hashimoto, SI, Nakatani, Y, Qu, W, Oshima, K, Tokunaga, K, Sugano, S, Hattori, M, Morishita, S & Matsushima, K 2011, 'Genome-wide profiling of DNA methylation in human cancer cells', Genomics, vol. 98, no. 4, pp. 280-287. https://doi.org/10.1016/j.ygeno.2011.07.003
Ogoshi K, Hashimoto SI, Nakatani Y, Qu W, Oshima K, Tokunaga K et al. Genome-wide profiling of DNA methylation in human cancer cells. Genomics. 2011 Oct;98(4):280-287. https://doi.org/10.1016/j.ygeno.2011.07.003
Ogoshi, Katsumi ; Hashimoto, Shin ichi ; Nakatani, Yoichiro ; Qu, Wei ; Oshima, Kenshiro ; Tokunaga, Katsushi ; Sugano, Sumio ; Hattori, Masahira ; Morishita, Shinichi ; Matsushima, Kouji. / Genome-wide profiling of DNA methylation in human cancer cells. In: Genomics. 2011 ; Vol. 98, No. 4. pp. 280-287.
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