Genome-wide survey of mutual homologous recombination in a highly sexual bacterial species

Koji Yahara, Mikihiko Kawai, Yoshikazu Furuta, Noriko Takahashi, Naofumi Handa, Takeshi Tsuru, Kenshiro Oshima, Masaru Yoshida, Takeshi Azuma, Masahira Hattori, Ikuo Uchiyama, Ichizo Kobayashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The nature of a species remains a fundamental and controversial question. The era of genome/metagenome sequencing has intensified the debate in prokaryotes because of extensive horizontal gene transfer. In this study, we conducted a genomewide survey of outcrossing homologous recombination in the highly sexual bacterial species Helicobacter pylori. We conducted multiple genome alignment and analyzed the entire data set of one-to-one orthologous genes for its global strains. We detected mosaic structures due to repeated recombination events and discordant phylogenies throughout the genomes of this species. Most of these genes including the "core" set of genes and horizontally transferred genes showed at least one recombination event. Taking into account the relationship between the nucleotide diversity and the minimum number of recombination events per nucleotide, we evaluated the recombination rate in every gene. The rate appears constant across the genome, but genes with a particularly high or low recombination rate were detected. Interestingly, genes with high recombination included those for DNA transformation and for basic cellular functions, such as biosynthesis and metabolism. Several highly divergent genes with a high recombination rate included those for host interaction, such as outer membrane proteins and lipopolysaccharide synthesis. These results provide a global picture of genome-wide distribution of outcrossing homologous recombination in a bacterial species for the first time, to our knowledge, and illustrate how a species can be shaped by mutual homologous recombination.

Original languageEnglish
Pages (from-to)628-640
Number of pages13
JournalGenome Biology and Evolution
Volume4
Issue number5
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Homologous Recombination
homologous recombination
recombination
Genetic Recombination
genome
Genome
gene
Genes
genes
outcrossing
Nucleotides
nucleotides
Metagenome
Horizontal Gene Transfer
Helicobacter pylori
Surveys and Questionnaires
outer membrane proteins
Phylogeny
prokaryotic cells
lipopolysaccharides

Keywords

  • Helicobacter pylori
  • Homologous recombination
  • Horizontal transfer
  • Population genomics
  • Species

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Yahara, K., Kawai, M., Furuta, Y., Takahashi, N., Handa, N., Tsuru, T., ... Kobayashi, I. (2012). Genome-wide survey of mutual homologous recombination in a highly sexual bacterial species. Genome Biology and Evolution, 4(5), 628-640. https://doi.org/10.1093/gbe/evs043

Genome-wide survey of mutual homologous recombination in a highly sexual bacterial species. / Yahara, Koji; Kawai, Mikihiko; Furuta, Yoshikazu; Takahashi, Noriko; Handa, Naofumi; Tsuru, Takeshi; Oshima, Kenshiro; Yoshida, Masaru; Azuma, Takeshi; Hattori, Masahira; Uchiyama, Ikuo; Kobayashi, Ichizo.

In: Genome Biology and Evolution, Vol. 4, No. 5, 2012, p. 628-640.

Research output: Contribution to journalArticle

Yahara, K, Kawai, M, Furuta, Y, Takahashi, N, Handa, N, Tsuru, T, Oshima, K, Yoshida, M, Azuma, T, Hattori, M, Uchiyama, I & Kobayashi, I 2012, 'Genome-wide survey of mutual homologous recombination in a highly sexual bacterial species', Genome Biology and Evolution, vol. 4, no. 5, pp. 628-640. https://doi.org/10.1093/gbe/evs043
Yahara, Koji ; Kawai, Mikihiko ; Furuta, Yoshikazu ; Takahashi, Noriko ; Handa, Naofumi ; Tsuru, Takeshi ; Oshima, Kenshiro ; Yoshida, Masaru ; Azuma, Takeshi ; Hattori, Masahira ; Uchiyama, Ikuo ; Kobayashi, Ichizo. / Genome-wide survey of mutual homologous recombination in a highly sexual bacterial species. In: Genome Biology and Evolution. 2012 ; Vol. 4, No. 5. pp. 628-640.
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