TY - JOUR
T1 - Genomic structure, expression, and transcriptional regulation of human Gal β1,3 GalNAc α2,3-sialyltransferase gene
AU - Taniguchi, Akiyoshi
AU - Morishima, Takashi
AU - Tsujita, Yuna
AU - Matsumoto, Yoshiko
AU - Matsumoto, Kojiro
N1 - Funding Information:
This work was supported in part by Grant No. 14572076 from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2003/1/10
Y1 - 2003/1/10
N2 - In this report, we describe transcriptional regulation of the human Gal β1,3 GalNAc α2,3-sialyltransferase II (hST3Gal II) gene. The results of 5′-RACE showed that the forms of two mRNAs differed only in the 5′-untranslated region (Types 1 and 2). According to analysis of the genomic structure, the transcriptional regulation of Type 1 and Type 2 mRNA isoforms depended on the p1 and p2 promoters, respectively. Both the mRNA isoforms were detected in various human tissues except colon, skeletal muscle, and peripheral blood leukocytes by RT-PCR analysis. In colon tissue, the Type 2 mRNA was detected, however, Type 1 mRNA was not detected. To elucidate the molecular basis of hST3Gal II gene expression, we isolated and characterized the function of the genomic region of hST3Gal II containing the p1 and p2 promoters. The activity of p2 promoter is much higher than that of the p1 promoter in the colon adenocarcinoma cell line, COLO205. These results suggest that the hST3Gal II gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of transcription.
AB - In this report, we describe transcriptional regulation of the human Gal β1,3 GalNAc α2,3-sialyltransferase II (hST3Gal II) gene. The results of 5′-RACE showed that the forms of two mRNAs differed only in the 5′-untranslated region (Types 1 and 2). According to analysis of the genomic structure, the transcriptional regulation of Type 1 and Type 2 mRNA isoforms depended on the p1 and p2 promoters, respectively. Both the mRNA isoforms were detected in various human tissues except colon, skeletal muscle, and peripheral blood leukocytes by RT-PCR analysis. In colon tissue, the Type 2 mRNA was detected, however, Type 1 mRNA was not detected. To elucidate the molecular basis of hST3Gal II gene expression, we isolated and characterized the function of the genomic region of hST3Gal II containing the p1 and p2 promoters. The activity of p2 promoter is much higher than that of the p1 promoter in the colon adenocarcinoma cell line, COLO205. These results suggest that the hST3Gal II gene is expressed specifically by alternative promoter utilization and is regulated in a tissue-restricted fashion at the level of transcription.
KW - Colon adenocarcinoma
KW - Sialyltransferase
KW - Transcriptional regulation
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U2 - 10.1016/S0006-291X(02)02899-1
DO - 10.1016/S0006-291X(02)02899-1
M3 - Article
C2 - 12504121
AN - SCOPUS:0037427494
SN - 0006-291X
VL - 300
SP - 570
EP - 576
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -