Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats

Yoichi Ohno, Koichi Matsuo, Hiromichi Suzuki, Hisao Tanase, Hiroko Ikeshima, Toshiya Takano, Takao Saruta

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To search for a genetic marker of the sarco(endo)plasmic reticulum Ca2+-dependent ATPase (SERCA) II gene in spontaneously hypertensive rats (SHRs) and to investigate differences in blood pressure and intracellular Ca2+ among some substrains of SHRs and Wistar-Kyoto (WKY) rats related to their SERCA II genotypes. Design and methods: The coding region of the SERCA II gene was sequenced in SHRs. Blood pressure and intracellular Ca2+ concentration ([Ca2+]i) in platelets were measured in substrains of SHRs and WKY rats with different SERCA II genotypes. Results: A point mutation that provided restriction fragment length polymorphisms (RFLPs) by HindIII or SauI was found in the SERCA II gene. The polymerase chain reaction (PCR) products were digested by HindIII in SHR substrains and WKY-Kyoto rats, whereas they were digested by SauI in normotensive strains and SHR-Toho. Among SHR-Kyoto, SHR-Toho, WKY-Kyoto and WKY-Charles River, the substrains with the HindIII-digested SERCA II genotype showed slightly but significantly higher systolic blood pressure and augmented agonist-stimulated [Ca2+]i than those with the SauI-digested genotype. Conclusions: RFLPs were found in the SERCA II gene. In the substrain analysis of SHRs and WKY rats, higher blood pressure and increased [Ca2+]i were associated with the SERCA II genotype digested by HindIII. The SERCA II gene locus has the potential to contribute to the development of hypertension and abnormal intracellular Ca2+ metabolism in SHRs. These RFLPs in the SERCA II gene should be a useful genetic marker.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalJournal of Hypertension
Volume14
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Reticulum
Calcium-Transporting ATPases
Inbred SHR Rats
Genotype
Genes
Inbred WKY Rats
Restriction Fragment Length Polymorphisms
Blood Pressure
Hypertension
Genetic Markers
Point Mutation
Rivers
Blood Platelets
Polymerase Chain Reaction

Keywords

  • Calcium metabolism
  • Restriction fragment length polymorphisms
  • Sarco(endo)plasmic reticulum Ca-dependent ATPase II gene
  • Spontaneously hypertensive rats

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine

Cite this

Ohno, Y., Matsuo, K., Suzuki, H., Tanase, H., Ikeshima, H., Takano, T., & Saruta, T. (1996). Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats. Journal of Hypertension, 14(3), 287-291.

Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats. / Ohno, Yoichi; Matsuo, Koichi; Suzuki, Hiromichi; Tanase, Hisao; Ikeshima, Hiroko; Takano, Toshiya; Saruta, Takao.

In: Journal of Hypertension, Vol. 14, No. 3, 1996, p. 287-291.

Research output: Contribution to journalArticle

Ohno, Y, Matsuo, K, Suzuki, H, Tanase, H, Ikeshima, H, Takano, T & Saruta, T 1996, 'Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats', Journal of Hypertension, vol. 14, no. 3, pp. 287-291.
Ohno, Yoichi ; Matsuo, Koichi ; Suzuki, Hiromichi ; Tanase, Hisao ; Ikeshima, Hiroko ; Takano, Toshiya ; Saruta, Takao. / Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats. In: Journal of Hypertension. 1996 ; Vol. 14, No. 3. pp. 287-291.
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T1 - Genotypes of sarco(endo)plasmic reticulum Ca2+-dependent ATPase II gene in substrains of spontaneously hypertensive rats

AU - Ohno, Yoichi

AU - Matsuo, Koichi

AU - Suzuki, Hiromichi

AU - Tanase, Hisao

AU - Ikeshima, Hiroko

AU - Takano, Toshiya

AU - Saruta, Takao

PY - 1996

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N2 - Objective: To search for a genetic marker of the sarco(endo)plasmic reticulum Ca2+-dependent ATPase (SERCA) II gene in spontaneously hypertensive rats (SHRs) and to investigate differences in blood pressure and intracellular Ca2+ among some substrains of SHRs and Wistar-Kyoto (WKY) rats related to their SERCA II genotypes. Design and methods: The coding region of the SERCA II gene was sequenced in SHRs. Blood pressure and intracellular Ca2+ concentration ([Ca2+]i) in platelets were measured in substrains of SHRs and WKY rats with different SERCA II genotypes. Results: A point mutation that provided restriction fragment length polymorphisms (RFLPs) by HindIII or SauI was found in the SERCA II gene. The polymerase chain reaction (PCR) products were digested by HindIII in SHR substrains and WKY-Kyoto rats, whereas they were digested by SauI in normotensive strains and SHR-Toho. Among SHR-Kyoto, SHR-Toho, WKY-Kyoto and WKY-Charles River, the substrains with the HindIII-digested SERCA II genotype showed slightly but significantly higher systolic blood pressure and augmented agonist-stimulated [Ca2+]i than those with the SauI-digested genotype. Conclusions: RFLPs were found in the SERCA II gene. In the substrain analysis of SHRs and WKY rats, higher blood pressure and increased [Ca2+]i were associated with the SERCA II genotype digested by HindIII. The SERCA II gene locus has the potential to contribute to the development of hypertension and abnormal intracellular Ca2+ metabolism in SHRs. These RFLPs in the SERCA II gene should be a useful genetic marker.

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