Ghrelin receptor in two species of anuran amphibian, bullfrog (rana catesbeiana), and japanese tree frog (hyla japonica)

Hiroyuki Kaiya, Yasushi Koizumi, Norifumi Konno, Kazutoshi Yamamoto, Minoru Uchiyama, Kenji Kangawa, Mikiya Miyazato

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    We have identified cDNA encoding a functional growth hormone secretagogue-receptor 1a (GHS-R1a, ghrelin receptor) in two species of anuran amphibian, the bullfrog (Rana cates-beiana), and the Japanese tree frog (Hyla japonica). Deduced receptor protein for bullfrog and Japanese tree frog (tree frog) was comprised of 374- and 371-amino acids, respectively.The two receptors shared 86% identity, and are grouped to the clade of the tetrapod homologs by phylogenetic analysis. In functional analyses, ghrelin and GHSR1a agonists increased intracellular Ca2+ concentration in GHS-R1a-transfected-HEK293 cell, but ligand selectivity of ghrelin with Ser3 and Thr3 was not observed between the two receptors. Bullfrog GHS-R1a mRNA was mainly expressed in the brain, stomach, and testis. In the brain, the gene expression was detected in the diencephalon and mesencephalon, but not in the pituitary. Tree frog GHS-R1a mRNA was predominantly expressed in the gastrointestinal tract and ovary, but not detected in the pituitary. In bullfrog stomach but not the brain, GHS-R1a mRNA expression increased after 10 days of fasting. For tree frog, GHS-R1a mRNA expression was increased in the brain, stomach and ventral skin by 10 days of fasting, and in the stomach and ventral skin by a dehydration treatment. Intracere-broventricular injection of ghrelin in dehydrated tree frog did not affect water absorption from the ventral skin.These results suggest that ghrelin is involved in energy homeostasis and possibly in osmoregulation in frogs.

    Original languageEnglish
    Article numberArticle 31
    JournalFrontiers in Endocrinology
    Volume2
    Issue numberSEP
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Ghrelin Receptor
    Rana catesbeiana
    Amphibians
    Anura
    Ghrelin
    Stomach
    Messenger RNA
    Brain
    Fasting
    Ranidae
    Skin Absorption
    Osmoregulation
    Diencephalon
    Skin
    HEK293 Cells
    Mesencephalon
    Dehydration
    Gastrointestinal Tract
    Testis
    Ovary

    Keywords

    • Bullfrog
    • Characterization
    • Cloning
    • Ghrelin
    • Growth hormone secretagogue-receptor 1a
    • Japanese tree frog

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism

    Cite this

    Kaiya, H., Koizumi, Y., Konno, N., Yamamoto, K., Uchiyama, M., Kangawa, K., & Miyazato, M. (2011). Ghrelin receptor in two species of anuran amphibian, bullfrog (rana catesbeiana), and japanese tree frog (hyla japonica). Frontiers in Endocrinology, 2(SEP), [Article 31]. https://doi.org/10.3389/fendo.2011.00031

    Ghrelin receptor in two species of anuran amphibian, bullfrog (rana catesbeiana), and japanese tree frog (hyla japonica). / Kaiya, Hiroyuki; Koizumi, Yasushi; Konno, Norifumi; Yamamoto, Kazutoshi; Uchiyama, Minoru; Kangawa, Kenji; Miyazato, Mikiya.

    In: Frontiers in Endocrinology, Vol. 2, No. SEP, Article 31, 2011.

    Research output: Contribution to journalArticle

    Kaiya, H, Koizumi, Y, Konno, N, Yamamoto, K, Uchiyama, M, Kangawa, K & Miyazato, M 2011, 'Ghrelin receptor in two species of anuran amphibian, bullfrog (rana catesbeiana), and japanese tree frog (hyla japonica)', Frontiers in Endocrinology, vol. 2, no. SEP, Article 31. https://doi.org/10.3389/fendo.2011.00031
    Kaiya, Hiroyuki ; Koizumi, Yasushi ; Konno, Norifumi ; Yamamoto, Kazutoshi ; Uchiyama, Minoru ; Kangawa, Kenji ; Miyazato, Mikiya. / Ghrelin receptor in two species of anuran amphibian, bullfrog (rana catesbeiana), and japanese tree frog (hyla japonica). In: Frontiers in Endocrinology. 2011 ; Vol. 2, No. SEP.
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    AU - Konno, Norifumi

    AU - Yamamoto, Kazutoshi

    AU - Uchiyama, Minoru

    AU - Kangawa, Kenji

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