Global gene repression by KaiC as a master process of prokaryotic circadian system

Yoichi Nakahira, Mitsunori Katayama, Hiroshi Miyashita, Shinsuke Kutsuna, Hideo Iwasaki, Tokitaka Oyama, Takao Kondo

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

A kaiABC clock gene cluster was previously identified from cyanobacterium Synechococcus elongatus PCC 7942, and the feedback regulation of kai genes was proposed as the core mechanism generating circadian oscillation. In this study, we confirmed that the Kai-based oscillator is the dominant circadian oscillator functioning in cyanobacteria. We probed the nature of this regulation and found that excess KaiC represses not only kaiBC but also the rhythmic components of all genes in the genome. This result strongly suggests that the KaiC protein primarily coordinates genomewide gene expression, including its own expression. We also found that a promoter derived from E. coli is feedback controlled by KaiC and restores the complete circadian rhythm in kaiBC-inactivated arrhythmic mutants, provided it can express kaiB and kaiC genes at an appropriate level. Unlike eukaryotic models, specific regulation of the kaiBC promoter is not essential for cyanobacterial circadian oscillations.

Original languageEnglish
Pages (from-to)881-885
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number3
DOIs
Publication statusPublished - 2004 Jan 20
Externally publishedYes

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Cyanobacteria
Synechococcus
Gene Components
Multigene Family
Circadian Rhythm
Genes
Genome
Escherichia coli
Gene Expression
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Global gene repression by KaiC as a master process of prokaryotic circadian system. / Nakahira, Yoichi; Katayama, Mitsunori; Miyashita, Hiroshi; Kutsuna, Shinsuke; Iwasaki, Hideo; Oyama, Tokitaka; Kondo, Takao.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 3, 20.01.2004, p. 881-885.

Research output: Contribution to journalArticle

Nakahira, Yoichi ; Katayama, Mitsunori ; Miyashita, Hiroshi ; Kutsuna, Shinsuke ; Iwasaki, Hideo ; Oyama, Tokitaka ; Kondo, Takao. / Global gene repression by KaiC as a master process of prokaryotic circadian system. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 3. pp. 881-885.
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