Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle

Takaki Tominaga, Sihui Ma, Kumiko Saitou, Katsuhiko Suzuki

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Carbohydrate (CHO) supplementation during exercise attenuates exercise-induced increases in plasma Interleukin (IL)-6 concentration. However, the effects of CHO supplementation on muscle IL-6 production during endurance exercise is controversial. The purpose of this study was to investigate the effects of CHO supplementation on muscle IL-6 production during endurance exercise with a special focus on the IL-6 producing cells. METHODS: C57BL/6J mice were divided into three groups-sedentary with water ingestion group as the control (Con; n = 10), exercise with water ingestion group (Ex; n = 10), and exercise with 6% glucose ingestion group (Ex + glucose; n = 10). The Ex and Ex + glucose groups completed 3 h of treadmill running (24 m/min, 7% incline) and were sacrificed immediately after exercise. RESULTS: The exercise-induced increases of plasma IL-6 concentration and gastrocnemius IL-6 gene expression were attenuated by glucose ingestion. However, the increases of soleus IL-6 gene expression and gastrocnemius and soleus IL-6 protein expression were not attenuated by glucose ingestion. Furthermore, we observed that macrophages that infiltrated muscle produce IL-6 and glucose ingestion attenuated the infiltration of IL-6-producing macrophages. CONCLUSION: This study revealed that infiltrating macrophages may be one type of IL-6-producing cells during endurance exercise, and the infiltration of these cells in muscle was attenuated by glucose ingestion. However, the effects of glucose ingestion on muscle IL-6 production were limited.

Original languageEnglish
JournalNutrients
Volume11
Issue number7
DOIs
Publication statusPublished - 2019 Jun 30

Fingerprint

interleukin-6
infiltration (hydrology)
Interleukin-6
exercise
macrophages
Eating
Macrophages
ingestion
Glucose
Muscles
muscles
glucose
mice
Gene Expression
gene expression
Water
exercise equipment
Inbred C57BL Mouse
Running
myocytes

Keywords

  • endurance exercise
  • glucose ingestion
  • interleukin 6 (IL-6)
  • macrophage
  • monocyte chemotactic protein (MCP)-1

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle. / Tominaga, Takaki; Ma, Sihui; Saitou, Kumiko; Suzuki, Katsuhiko.

In: Nutrients, Vol. 11, No. 7, 30.06.2019.

Research output: Contribution to journalArticle

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abstract = "BACKGROUND: Carbohydrate (CHO) supplementation during exercise attenuates exercise-induced increases in plasma Interleukin (IL)-6 concentration. However, the effects of CHO supplementation on muscle IL-6 production during endurance exercise is controversial. The purpose of this study was to investigate the effects of CHO supplementation on muscle IL-6 production during endurance exercise with a special focus on the IL-6 producing cells. METHODS: C57BL/6J mice were divided into three groups-sedentary with water ingestion group as the control (Con; n = 10), exercise with water ingestion group (Ex; n = 10), and exercise with 6{\%} glucose ingestion group (Ex + glucose; n = 10). The Ex and Ex + glucose groups completed 3 h of treadmill running (24 m/min, 7{\%} incline) and were sacrificed immediately after exercise. RESULTS: The exercise-induced increases of plasma IL-6 concentration and gastrocnemius IL-6 gene expression were attenuated by glucose ingestion. However, the increases of soleus IL-6 gene expression and gastrocnemius and soleus IL-6 protein expression were not attenuated by glucose ingestion. Furthermore, we observed that macrophages that infiltrated muscle produce IL-6 and glucose ingestion attenuated the infiltration of IL-6-producing macrophages. CONCLUSION: This study revealed that infiltrating macrophages may be one type of IL-6-producing cells during endurance exercise, and the infiltration of these cells in muscle was attenuated by glucose ingestion. However, the effects of glucose ingestion on muscle IL-6 production were limited.",
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