Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals

Takuya Miyagawa, Toshinori Fujie, Ferdinandus, Tat Thang Vo Doan, Hirotaka Sato, Shinji Takeoka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal's muscular activity. Herein, we report super flexible and self-adhesive (no need of glue) nanothermosensor consisting of stacked two different polymeric nanosheets with thermosensitive (Eu-tris (dinaphthoylmethane)-bis-trioctylphosphine oxide: EuDT) and insensitive (Rhodamine 800) dyes being embedded. Such stacked nanosheets allow for the ratiometric thermometry, with which the undesired luminescence intensity shift due to focal drift or animal's z-axis displacement is eliminated and the desired intensity shift solely due to the temperature shift of the sample (living muscle) can be acquired. With the stacked luminescent nanosheets, we achieved the first-ever demonstration of video filming of chronologically changing temperature-shift distribution from the rest state to the active state of the muscles in the living animal. The polymer nanosheet engineering and in vivo microthermography presented in the paper are promising technologies to microscopically explore the heat production and heat transfer in living cells, tissues, and organisms with high spatial resolution beyond what existing thermometric technologies such as infrared thermography have ever achieved.

Original languageEnglish
Pages (from-to)33377-33385
Number of pages9
JournalACS Applied Materials and Interfaces
Volume8
Issue number49
DOIs
Publication statusPublished - 2016 Dec 14

Fingerprint

Glues
Nanosheets
Animals
Muscle
Temperature
Ultrathin films
Polymer films
Luminescence
Adhesives
Polymers
Coloring Agents
Demonstrations
Dyes
Cells
Tissue
Heat transfer
Molecules
Oxides

Keywords

  • heat production
  • luminescent
  • optical sensor
  • thermometry
  • ultrathin film (nanosheet)

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals. / Miyagawa, Takuya; Fujie, Toshinori; Ferdinandus; Vo Doan, Tat Thang; Sato, Hirotaka; Takeoka, Shinji.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 49, 14.12.2016, p. 33377-33385.

Research output: Contribution to journalArticle

Miyagawa, Takuya ; Fujie, Toshinori ; Ferdinandus ; Vo Doan, Tat Thang ; Sato, Hirotaka ; Takeoka, Shinji. / Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 49. pp. 33377-33385.
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