Abstract
A photobioreactor was constructed in the form of a Perspex column 900 mm tall with an internal diameter of 70 mm. The reactor volume was 1.8 L and the light source consisted of a metal-halide lamp to reproduce sunlight. Light was distributed through the culture using a new type of optical fiber that diffuses light out through its surface, perpendicular to the fiber axis. A cluster of 661 light-diffusing optical fibers (LDOFs) pass from the light source through the reactor column (60-cm culture depth) and are connected to a mirror at the top of the reactor. This biosolar reactor has been used for the production of glutamate from CO2 by the marine cyanobacterium Synechococcus sp. NKBG040607. We present here details of the construction of the biosolar reactor and characterization of its properties. The effect of light intensity on glutamate production was measured. Carbon dioxide-to-glutamate conversion ratios were determined at different cell densities: the maximum conversion ratio (28%) was achieved at a cell density of 3x108 cells/mL. A comparison of glutamate production using the LDOF biosolar reactor described here with production by batch culture using free or immobilized cells showed that use of an optical-fiber biosolar reactor increased glutamate-production efficiency 6.75-fold. We conclude that as a result of its high surface-to-volume ratio (692/m) increased photoproduction of useful compounds may be achieved. Such a system is generally applicable to all aspects of photobiotechnology.
| Original language | English |
|---|---|
| Pages (from-to) | 157-167 |
| Number of pages | 11 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 28-29 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1991 Mar |
| Externally published | Yes |
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Keywords
- biosolar reactor
- Glutamate
- marine cyanobacteria
- optical fiber
- Synechococcus
ASJC Scopus subject areas
- Bioengineering
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Applied Microbiology and Biotechnology
- Environmental Engineering
- Molecular Biology
Cite this
Glutamate production from CO2 by Marine Cyanobacterium Synechococcus sp. - Using a Novel Biosolar Reactor Employing Light-Diffusing Optical Fibers. / Matsunaga, Tadashi; Takeyama, Haruko; Sudo, Hiroaki; Oyama, Nobuo; Ariura, Shunsuke; Takano, Hiroyuki; Hirano, Morio; Burgess, J. Grant; Sode, Koji; Nakamura, Noriyuki.
In: Applied Biochemistry and Biotechnology, Vol. 28-29, No. 1, 03.1991, p. 157-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glutamate production from CO2 by Marine Cyanobacterium Synechococcus sp. - Using a Novel Biosolar Reactor Employing Light-Diffusing Optical Fibers
AU - Matsunaga, Tadashi
AU - Takeyama, Haruko
AU - Sudo, Hiroaki
AU - Oyama, Nobuo
AU - Ariura, Shunsuke
AU - Takano, Hiroyuki
AU - Hirano, Morio
AU - Burgess, J. Grant
AU - Sode, Koji
AU - Nakamura, Noriyuki
PY - 1991/3
Y1 - 1991/3
N2 - A photobioreactor was constructed in the form of a Perspex column 900 mm tall with an internal diameter of 70 mm. The reactor volume was 1.8 L and the light source consisted of a metal-halide lamp to reproduce sunlight. Light was distributed through the culture using a new type of optical fiber that diffuses light out through its surface, perpendicular to the fiber axis. A cluster of 661 light-diffusing optical fibers (LDOFs) pass from the light source through the reactor column (60-cm culture depth) and are connected to a mirror at the top of the reactor. This biosolar reactor has been used for the production of glutamate from CO2 by the marine cyanobacterium Synechococcus sp. NKBG040607. We present here details of the construction of the biosolar reactor and characterization of its properties. The effect of light intensity on glutamate production was measured. Carbon dioxide-to-glutamate conversion ratios were determined at different cell densities: the maximum conversion ratio (28%) was achieved at a cell density of 3x108 cells/mL. A comparison of glutamate production using the LDOF biosolar reactor described here with production by batch culture using free or immobilized cells showed that use of an optical-fiber biosolar reactor increased glutamate-production efficiency 6.75-fold. We conclude that as a result of its high surface-to-volume ratio (692/m) increased photoproduction of useful compounds may be achieved. Such a system is generally applicable to all aspects of photobiotechnology.
AB - A photobioreactor was constructed in the form of a Perspex column 900 mm tall with an internal diameter of 70 mm. The reactor volume was 1.8 L and the light source consisted of a metal-halide lamp to reproduce sunlight. Light was distributed through the culture using a new type of optical fiber that diffuses light out through its surface, perpendicular to the fiber axis. A cluster of 661 light-diffusing optical fibers (LDOFs) pass from the light source through the reactor column (60-cm culture depth) and are connected to a mirror at the top of the reactor. This biosolar reactor has been used for the production of glutamate from CO2 by the marine cyanobacterium Synechococcus sp. NKBG040607. We present here details of the construction of the biosolar reactor and characterization of its properties. The effect of light intensity on glutamate production was measured. Carbon dioxide-to-glutamate conversion ratios were determined at different cell densities: the maximum conversion ratio (28%) was achieved at a cell density of 3x108 cells/mL. A comparison of glutamate production using the LDOF biosolar reactor described here with production by batch culture using free or immobilized cells showed that use of an optical-fiber biosolar reactor increased glutamate-production efficiency 6.75-fold. We conclude that as a result of its high surface-to-volume ratio (692/m) increased photoproduction of useful compounds may be achieved. Such a system is generally applicable to all aspects of photobiotechnology.
KW - biosolar reactor
KW - Glutamate
KW - marine cyanobacteria
KW - optical fiber
KW - Synechococcus
UR - http://www.scopus.com/inward/record.url?scp=0001251794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001251794&partnerID=8YFLogxK
U2 - 10.1007/BF02922597
DO - 10.1007/BF02922597
M3 - Article
VL - 28-29
SP - 157
EP - 167
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
SN - 0273-2289
IS - 1
ER -