Glutamate release from astrocytes is stimulated via the appearance of exocytosis during cyclic AMP-induced morphologic changes

Hatsuki Shiga, Jun Murakami, Takashi Nagao, Motoki Tanaka, Koichi Kawahara, Ichiro Matsuoka, Etsuro Ito

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies have shown that astrocytes release various transmitters including glutamate and thus directly affect synaptic neurotransmission. The mechanisms involved in the release of glutamate from astrocytes remain unclear, however. In the present study, we examined differences in 1) the amount of glutamate released, 2) the appearance of exocytosis, and 3) the expression of SNARE (soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor) proteins between cyclic AMP-treated and non-treated astrocytes in culture. Extracellular glutamate was detected in the recording solution of cyclic AMP-treated astrocytes after stimulation with ATP by high-performance liquid chromatography and NADH imaging. Exocytosis, which was observed by FM1-43 imaging, appeared in cyclic AMP-treated astrocytes in a punctiform fashion, but not in non-treated cells, after stimulation with ATP and glutamate. Immunocytochemistry and Western blotting showed that the amount of SNARE proteins increased during cAMP-induced morphologic changes, and in particular, a v-SNARE, synaptobrevin, appeared as punctiform staining in the cytosol of cyclic AMP-treated astrocytes. These findings show that astrocytes acquire SNARE proteins during cyclic AMP-induced differentiation, and suggest that glutamate is released by exocytosis in cyclic AMP-treated astrocytes in response to ATP released from neighboring neurons and astrocytes.

Original languageEnglish
Pages (from-to)338-347
Number of pages10
JournalJournal of Neuroscience Research
Volume84
Issue number2
DOIs
Publication statusPublished - 2006 Aug 1
Externally publishedYes

Fingerprint

Exocytosis
Astrocytes
Cyclic AMP
Glutamic Acid
SNARE Proteins
Adenosine Triphosphate
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
R-SNARE Proteins
Cyclic AMP Receptor Protein
Synaptic Transmission
NAD
Cytosol
Western Blotting
Immunohistochemistry
High Pressure Liquid Chromatography
Staining and Labeling
Neurons

Keywords

  • ATP
  • FM1-43 imaging
  • Gliotransmitter
  • HPLC
  • NADH imaging
  • SNARE protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Glutamate release from astrocytes is stimulated via the appearance of exocytosis during cyclic AMP-induced morphologic changes. / Shiga, Hatsuki; Murakami, Jun; Nagao, Takashi; Tanaka, Motoki; Kawahara, Koichi; Matsuoka, Ichiro; Ito, Etsuro.

In: Journal of Neuroscience Research, Vol. 84, No. 2, 01.08.2006, p. 338-347.

Research output: Contribution to journalArticle

Shiga, Hatsuki ; Murakami, Jun ; Nagao, Takashi ; Tanaka, Motoki ; Kawahara, Koichi ; Matsuoka, Ichiro ; Ito, Etsuro. / Glutamate release from astrocytes is stimulated via the appearance of exocytosis during cyclic AMP-induced morphologic changes. In: Journal of Neuroscience Research. 2006 ; Vol. 84, No. 2. pp. 338-347.
@article{28dab7309d2f4e6284d1f1ecc3b6e551,
title = "Glutamate release from astrocytes is stimulated via the appearance of exocytosis during cyclic AMP-induced morphologic changes",
abstract = "Recent studies have shown that astrocytes release various transmitters including glutamate and thus directly affect synaptic neurotransmission. The mechanisms involved in the release of glutamate from astrocytes remain unclear, however. In the present study, we examined differences in 1) the amount of glutamate released, 2) the appearance of exocytosis, and 3) the expression of SNARE (soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor) proteins between cyclic AMP-treated and non-treated astrocytes in culture. Extracellular glutamate was detected in the recording solution of cyclic AMP-treated astrocytes after stimulation with ATP by high-performance liquid chromatography and NADH imaging. Exocytosis, which was observed by FM1-43 imaging, appeared in cyclic AMP-treated astrocytes in a punctiform fashion, but not in non-treated cells, after stimulation with ATP and glutamate. Immunocytochemistry and Western blotting showed that the amount of SNARE proteins increased during cAMP-induced morphologic changes, and in particular, a v-SNARE, synaptobrevin, appeared as punctiform staining in the cytosol of cyclic AMP-treated astrocytes. These findings show that astrocytes acquire SNARE proteins during cyclic AMP-induced differentiation, and suggest that glutamate is released by exocytosis in cyclic AMP-treated astrocytes in response to ATP released from neighboring neurons and astrocytes.",
keywords = "ATP, FM1-43 imaging, Gliotransmitter, HPLC, NADH imaging, SNARE protein",
author = "Hatsuki Shiga and Jun Murakami and Takashi Nagao and Motoki Tanaka and Koichi Kawahara and Ichiro Matsuoka and Etsuro Ito",
year = "2006",
month = "8",
day = "1",
doi = "10.1002/jnr.20885",
language = "English",
volume = "84",
pages = "338--347",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Glutamate release from astrocytes is stimulated via the appearance of exocytosis during cyclic AMP-induced morphologic changes

AU - Shiga, Hatsuki

AU - Murakami, Jun

AU - Nagao, Takashi

AU - Tanaka, Motoki

AU - Kawahara, Koichi

AU - Matsuoka, Ichiro

AU - Ito, Etsuro

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Recent studies have shown that astrocytes release various transmitters including glutamate and thus directly affect synaptic neurotransmission. The mechanisms involved in the release of glutamate from astrocytes remain unclear, however. In the present study, we examined differences in 1) the amount of glutamate released, 2) the appearance of exocytosis, and 3) the expression of SNARE (soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor) proteins between cyclic AMP-treated and non-treated astrocytes in culture. Extracellular glutamate was detected in the recording solution of cyclic AMP-treated astrocytes after stimulation with ATP by high-performance liquid chromatography and NADH imaging. Exocytosis, which was observed by FM1-43 imaging, appeared in cyclic AMP-treated astrocytes in a punctiform fashion, but not in non-treated cells, after stimulation with ATP and glutamate. Immunocytochemistry and Western blotting showed that the amount of SNARE proteins increased during cAMP-induced morphologic changes, and in particular, a v-SNARE, synaptobrevin, appeared as punctiform staining in the cytosol of cyclic AMP-treated astrocytes. These findings show that astrocytes acquire SNARE proteins during cyclic AMP-induced differentiation, and suggest that glutamate is released by exocytosis in cyclic AMP-treated astrocytes in response to ATP released from neighboring neurons and astrocytes.

AB - Recent studies have shown that astrocytes release various transmitters including glutamate and thus directly affect synaptic neurotransmission. The mechanisms involved in the release of glutamate from astrocytes remain unclear, however. In the present study, we examined differences in 1) the amount of glutamate released, 2) the appearance of exocytosis, and 3) the expression of SNARE (soluble N-ethylmaleimide sensitive fusion protein attachment protein receptor) proteins between cyclic AMP-treated and non-treated astrocytes in culture. Extracellular glutamate was detected in the recording solution of cyclic AMP-treated astrocytes after stimulation with ATP by high-performance liquid chromatography and NADH imaging. Exocytosis, which was observed by FM1-43 imaging, appeared in cyclic AMP-treated astrocytes in a punctiform fashion, but not in non-treated cells, after stimulation with ATP and glutamate. Immunocytochemistry and Western blotting showed that the amount of SNARE proteins increased during cAMP-induced morphologic changes, and in particular, a v-SNARE, synaptobrevin, appeared as punctiform staining in the cytosol of cyclic AMP-treated astrocytes. These findings show that astrocytes acquire SNARE proteins during cyclic AMP-induced differentiation, and suggest that glutamate is released by exocytosis in cyclic AMP-treated astrocytes in response to ATP released from neighboring neurons and astrocytes.

KW - ATP

KW - FM1-43 imaging

KW - Gliotransmitter

KW - HPLC

KW - NADH imaging

KW - SNARE protein

UR - http://www.scopus.com/inward/record.url?scp=33746405156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746405156&partnerID=8YFLogxK

U2 - 10.1002/jnr.20885

DO - 10.1002/jnr.20885

M3 - Article

VL - 84

SP - 338

EP - 347

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 2

ER -