Glutamatergic neurotransmission in the procerebrum (Olfactory Center) of a terrestrial mollusk

Ryota Matsuo, Suguru Kobayashi, Satoshi Watanabe, Shigeyuki Namiki, Sho Iinuma, Hirokazu Sakamoto, Kenzo Hirose, Etsuro Ito

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The terrestrial slug Limax has the ability to learn odor associations. This ability depends on the function of the procerebrum, the secondary olfactory center in the brain. Among the various neurotransmitters that are thought to be involved in the function of the procerebrum, glutamate is one of the most important molecules. However, the existence and function of glutamate in this system have been proposed solely on the basis of a few lines of indirect evidence from pharmacological experiments. In the present study, we demonstrated the existence and release of glutamate as a neurotransmitter in the procerebrum of Limax, by using three different techniques: 1) immunohistochemistry of glutamate, 2) in situ hybridization to mRNA of the vesicular glutamate transporter, and 3) real-time imaging of glutamate release within the procerebrum using the glutamate optical sensor EOS2. The release of glutamate within the cell mass layer of the procerebrum was synchronized with oscillation of the local field potential and had the same physiological properties as this oscillation; both were blocked by a serotonin antagonist and were propagated in an apical to basal direction in the procerebrum. Our observations suggest strongly that the oscillation of the local field potential is driven by the glutamate released by bursting neurons in the procerebrum.

Original languageEnglish
Pages (from-to)3011-3023
Number of pages13
JournalJournal of Neuroscience Research
Volume87
Issue number13
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

Fingerprint

Mollusca
Synaptic Transmission
Glutamic Acid
Neurotransmitter Agents
Vesicular Glutamate Transport Proteins
Serotonin Antagonists
Gastropoda
In Situ Hybridization
Immunohistochemistry
Pharmacology
Neurons
Messenger RNA
Brain

Keywords

  • Glutamate sensor
  • Glutamate transporter
  • Limax
  • Local field potential
  • Oscillation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Glutamatergic neurotransmission in the procerebrum (Olfactory Center) of a terrestrial mollusk. / Matsuo, Ryota; Kobayashi, Suguru; Watanabe, Satoshi; Namiki, Shigeyuki; Iinuma, Sho; Sakamoto, Hirokazu; Hirose, Kenzo; Ito, Etsuro.

In: Journal of Neuroscience Research, Vol. 87, No. 13, 10.2009, p. 3011-3023.

Research output: Contribution to journalArticle

Matsuo, R, Kobayashi, S, Watanabe, S, Namiki, S, Iinuma, S, Sakamoto, H, Hirose, K & Ito, E 2009, 'Glutamatergic neurotransmission in the procerebrum (Olfactory Center) of a terrestrial mollusk', Journal of Neuroscience Research, vol. 87, no. 13, pp. 3011-3023. https://doi.org/10.1002/jnr.22130
Matsuo, Ryota ; Kobayashi, Suguru ; Watanabe, Satoshi ; Namiki, Shigeyuki ; Iinuma, Sho ; Sakamoto, Hirokazu ; Hirose, Kenzo ; Ito, Etsuro. / Glutamatergic neurotransmission in the procerebrum (Olfactory Center) of a terrestrial mollusk. In: Journal of Neuroscience Research. 2009 ; Vol. 87, No. 13. pp. 3011-3023.
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