Glycosylceramides from marine green microalga Tetraselmis sp.

Atsushi Arakaki; Daisuke Iwama; Yue Liang; Nagisa Murakami; Masaharu Ishikura; Tsuyoshi Tanaka; Tadashi Matsunaga

doi:10.1016/j.phytochem.2012.09.006

Glycosylceramides from marine green microalga Tetraselmis sp.

Atsushi Arakaki, Daisuke Iwama, Yue Liang, Nagisa Murakami, Masaharu Ishikura, Tsuyoshi Tanaka, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.

Original language	English
Pages (from-to)	107-114
Number of pages	8
Journal	Phytochemistry
Volume	85
DOIs	https://doi.org/10.1016/j.phytochem.2012.09.006
Publication status	Published - 2013 Jan
Externally published	Yes

Keywords

Δ3-unsaturatation
Glucosylceramides
Prasinophyceae
Sphingolipids
Tetraselmis sp.

ASJC Scopus subject areas

Plant Science
Biochemistry
Molecular Biology
Horticulture

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title = "Glycosylceramides from marine green microalga Tetraselmis sp.",

abstract = "Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.",

keywords = "Δ3-unsaturatation, Glucosylceramides, Prasinophyceae, Sphingolipids, Tetraselmis sp.",

author = "Atsushi Arakaki and Daisuke Iwama and Yue Liang and Nagisa Murakami and Masaharu Ishikura and Tsuyoshi Tanaka and Tadashi Matsunaga",

year = "2013",

month = jan,

doi = "10.1016/j.phytochem.2012.09.006",

language = "English",

volume = "85",

pages = "107--114",

journal = "Phytochemistry",

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publisher = "Elsevier Limited",

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T1 - Glycosylceramides from marine green microalga Tetraselmis sp.

AU - Arakaki, Atsushi

AU - Iwama, Daisuke

AU - Liang, Yue

AU - Murakami, Nagisa

AU - Ishikura, Masaharu

AU - Tanaka, Tsuyoshi

AU - Matsunaga, Tadashi

PY - 2013/1

Y1 - 2013/1

N2 - Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.

AB - Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.

KW - Δ3-unsaturatation

KW - Glucosylceramides

KW - Prasinophyceae

KW - Sphingolipids

KW - Tetraselmis sp.

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