Abstract
Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.
| Original language | English |
|---|---|
| Pages (from-to) | 107-114 |
| Number of pages | 8 |
| Journal | Phytochemistry |
| Volume | 85 |
| DOIs | |
| Publication status | Published - 2013 Jan |
| Externally published | Yes |
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Keywords
- Δ3-unsaturatation
- Glucosylceramides
- Prasinophyceae
- Sphingolipids
- Tetraselmis sp.
ASJC Scopus subject areas
- Plant Science
- Biochemistry
- Molecular Biology
- Horticulture
Cite this
Glycosylceramides from marine green microalga Tetraselmis sp. / Arakaki, Atsushi; Iwama, Daisuke; Liang, Yue; Murakami, Nagisa; Ishikura, Masaharu; Tanaka, Tsuyoshi; Matsunaga, Tadashi.
In: Phytochemistry, Vol. 85, 01.2013, p. 107-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glycosylceramides from marine green microalga Tetraselmis sp.
AU - Arakaki, Atsushi
AU - Iwama, Daisuke
AU - Liang, Yue
AU - Murakami, Nagisa
AU - Ishikura, Masaharu
AU - Tanaka, Tsuyoshi
AU - Matsunaga, Tadashi
PY - 2013/1
Y1 - 2013/1
N2 - Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.
AB - Glycosylceramides are ubiquitous and important components of the plasma membrane in most eukaryotic cells and a few bacteria. They play significant roles in a variety of cellular functions. Their molecular structures are well recognized in animals, higher plants, and fungi, but are poorly characterized in lower plants. In this study, a high glycosylceramide-producing microalgal strain Tetraselmis sp. NKG 400013 was found. TLC and MS analyses established the presence of glycosylceramides, GT1 and GT2, in this strain. Their chemical structures were determined by NMR spectroscopy and GC/MS, and were identified as glycosylceramides consisting of the typical botanical sphingoid base ([4E, 8E]-sphinga-4, 8-dienine) and 2-hydroxy-Δ3-unsaturated fatty acyl chains, respectively. To our knowledge, the occurrence of glycosylceramides in microalga of the class Prasinophyceae was previously unknown.
KW - Δ3-unsaturatation
KW - Glucosylceramides
KW - Prasinophyceae
KW - Sphingolipids
KW - Tetraselmis sp.
UR - http://www.scopus.com/inward/record.url?scp=84871319087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871319087&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2012.09.006
DO - 10.1016/j.phytochem.2012.09.006
M3 - Article
VL - 85
SP - 107
EP - 114
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
ER -