Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system

Daxiang Cui, Hong Zhang, Kan Wang, Feng Gao, Xueqing Zhang, Toru Asahi, Rong He, Tetsuya Osaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalNano Biomedicine and Engineering
Volume3
Issue number2
Publication statusPublished - 2011

Fingerprint

Transcription
Genes
Gold
Nanoparticles
Proteins
Bioactivity
Innovation
Fabrication
Catalysts

Keywords

  • Efficiency
  • Gold nanoparticle
  • Rapid translation system

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system. / Cui, Daxiang; Zhang, Hong; Wang, Kan; Gao, Feng; Zhang, Xueqing; Asahi, Toru; He, Rong; Osaka, Tetsuya.

In: Nano Biomedicine and Engineering, Vol. 3, No. 2, 2011, p. 120-125.

Research output: Contribution to journalArticle

Cui, Daxiang ; Zhang, Hong ; Wang, Kan ; Gao, Feng ; Zhang, Xueqing ; Asahi, Toru ; He, Rong ; Osaka, Tetsuya. / Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system. In: Nano Biomedicine and Engineering. 2011 ; Vol. 3, No. 2. pp. 120-125.
@article{126c79678392402a98a3f3f391339be7,
title = "Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system",
abstract = "Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30{\%} and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.",
keywords = "Efficiency, Gold nanoparticle, Rapid translation system",
author = "Daxiang Cui and Hong Zhang and Kan Wang and Feng Gao and Xueqing Zhang and Toru Asahi and Rong He and Tetsuya Osaka",
year = "2011",
language = "English",
volume = "3",
pages = "120--125",
journal = "Nano Biomedicine and Engineering",
issn = "2150-5578",
publisher = "Open Access House of Science and Technology (OAHOST)",
number = "2",

}

TY - JOUR

T1 - Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system

AU - Cui, Daxiang

AU - Zhang, Hong

AU - Wang, Kan

AU - Gao, Feng

AU - Zhang, Xueqing

AU - Asahi, Toru

AU - He, Rong

AU - Osaka, Tetsuya

PY - 2011

Y1 - 2011

N2 - Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

AB - Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

KW - Efficiency

KW - Gold nanoparticle

KW - Rapid translation system

UR - http://www.scopus.com/inward/record.url?scp=80053440358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053440358&partnerID=8YFLogxK

M3 - Article

VL - 3

SP - 120

EP - 125

JO - Nano Biomedicine and Engineering

JF - Nano Biomedicine and Engineering

SN - 2150-5578

IS - 2

ER -