Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system

Daxiang Cui, Hong Zhang, Kan Wang, Feng Gao, Xueqing Zhang, Toru Asahi, Rong He, Tetsuya Osaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Herein we report that the in vitro gene transcription-translation efficiency can be dramatically enhanced by gold nanoparticles of 5nm in diameter. The addition of less than or equal to 1.2 nM of gold nanoparticles of 5 nm in diameter into rapid-translation-system (RTS) reagents increased the transcription-translation efficiency up to 30% and shortened the reaction time to 4 h, with the same or higher translation yields. Gold nanoparticles did not decrease the yields' bioactivity. The results show that gold nanoparticles of 5 nm may act as a bio-catalyst in the RTS reaction. This innovation has great potential in applications such as large-scale protein fabrication, gene transcription-translation regulation, and studies of structure and function of toxic protein.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalNano Biomedicine and Engineering
Volume3
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • Efficiency
  • Gold nanoparticle
  • Rapid translation system

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Gold nanoparticles enhance efficiency of in vitro gene transcription-translation system'. Together they form a unique fingerprint.

  • Cite this