Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks

Betty McConn, Guoqing Wang, Jiaqing Yi, Elizabeth R. Gilbert, Tomohiro Osugi, Takayoshi Ubuka, Kazuyoshi Tsutsui, Vishwajit S. Chowdhury, Mitsuhiro Furuse, Mark A. Cline

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Gonadotropin-inhibitory hormone (GnIH), a 12 amino acid peptide, is expressed in the avian brain and inhibits luteinizing hormone secretion. Additionally, exogenous injection of GnIH causes increased food intake of chicks although the central mechanism mediating this response is poorly understood. Hence, the purpose of our study was to elucidate the central mechanism of the GnIH orexigenic response using 12 day post hatch layer-type chicks as models. Firstly, via mass spectrometry we deduced the chicken GnIH amino acid sequence: SIRPSAYLPLRFamide. Following this we used chicken GnIH to demonstrate that intracerebroventricular (ICV) injection of 2.6 and 7.8 nmol causes increased food intake up to 150 min following injection with no effect on water intake. The number of c-Fos immunoreactive cells was quantified in appetite-associated hypothalamic nuclei following ICV GnIH and only the lateral hypothalamic area (LHA) had an increase of c-Fos positive neurons. From whole hypothalamus samples following ICV GnIH injection abundance of several appetite-associated mRNA was quantified which demonstrated that mRNA for neuropeptide Y (NPY) was increased while mRNA for proopiomelanocortin (POMC) was decreased. This was not the case for mRNA abundance in isolated LHA where NPY and POMC were not affected but melanin-concentrating hormone (MCH) mRNA was increased. A comprehensive behavior analysis was conducted after ICV GnIH injection which demonstrated a variety of behaviors unrelated to appetite were affected. In sum, these results implicate activation of the LHA in the GnIH orexigenic response and NPY, POMC and MCH are likely also involved.

    Original languageEnglish
    Pages (from-to)327-334
    Number of pages8
    JournalNeuropeptides
    Volume48
    Issue number6
    DOIs
    Publication statusPublished - 2014 Dec 1

    Fingerprint

    Gonadotropins
    Eating
    Hormones
    Lateral Hypothalamic Area
    Pro-Opiomelanocortin
    Neuropeptide Y
    Appetite
    Messenger RNA
    Injections
    Chickens
    Luteinizing Hormone
    Hypothalamus
    Drinking
    Amino Acid Sequence
    Mass Spectrometry
    Neurons
    Amino Acids
    Peptides
    Brain

    Keywords

    • Chick
    • Gonadotropin-inhibitory hormone
    • Hypothalamus

    ASJC Scopus subject areas

    • Endocrinology
    • Neurology
    • Cellular and Molecular Neuroscience
    • Endocrine and Autonomic Systems

    Cite this

    McConn, B., Wang, G., Yi, J., Gilbert, E. R., Osugi, T., Ubuka, T., ... Cline, M. A. (2014). Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. Neuropeptides, 48(6), 327-334. https://doi.org/10.1016/j.npep.2014.09.001

    Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. / McConn, Betty; Wang, Guoqing; Yi, Jiaqing; Gilbert, Elizabeth R.; Osugi, Tomohiro; Ubuka, Takayoshi; Tsutsui, Kazuyoshi; Chowdhury, Vishwajit S.; Furuse, Mitsuhiro; Cline, Mark A.

    In: Neuropeptides, Vol. 48, No. 6, 01.12.2014, p. 327-334.

    Research output: Contribution to journalArticle

    McConn, B, Wang, G, Yi, J, Gilbert, ER, Osugi, T, Ubuka, T, Tsutsui, K, Chowdhury, VS, Furuse, M & Cline, MA 2014, 'Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks', Neuropeptides, vol. 48, no. 6, pp. 327-334. https://doi.org/10.1016/j.npep.2014.09.001
    McConn, Betty ; Wang, Guoqing ; Yi, Jiaqing ; Gilbert, Elizabeth R. ; Osugi, Tomohiro ; Ubuka, Takayoshi ; Tsutsui, Kazuyoshi ; Chowdhury, Vishwajit S. ; Furuse, Mitsuhiro ; Cline, Mark A. / Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. In: Neuropeptides. 2014 ; Vol. 48, No. 6. pp. 327-334.
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    abstract = "Gonadotropin-inhibitory hormone (GnIH), a 12 amino acid peptide, is expressed in the avian brain and inhibits luteinizing hormone secretion. Additionally, exogenous injection of GnIH causes increased food intake of chicks although the central mechanism mediating this response is poorly understood. Hence, the purpose of our study was to elucidate the central mechanism of the GnIH orexigenic response using 12 day post hatch layer-type chicks as models. Firstly, via mass spectrometry we deduced the chicken GnIH amino acid sequence: SIRPSAYLPLRFamide. Following this we used chicken GnIH to demonstrate that intracerebroventricular (ICV) injection of 2.6 and 7.8 nmol causes increased food intake up to 150 min following injection with no effect on water intake. The number of c-Fos immunoreactive cells was quantified in appetite-associated hypothalamic nuclei following ICV GnIH and only the lateral hypothalamic area (LHA) had an increase of c-Fos positive neurons. From whole hypothalamus samples following ICV GnIH injection abundance of several appetite-associated mRNA was quantified which demonstrated that mRNA for neuropeptide Y (NPY) was increased while mRNA for proopiomelanocortin (POMC) was decreased. This was not the case for mRNA abundance in isolated LHA where NPY and POMC were not affected but melanin-concentrating hormone (MCH) mRNA was increased. A comprehensive behavior analysis was conducted after ICV GnIH injection which demonstrated a variety of behaviors unrelated to appetite were affected. In sum, these results implicate activation of the LHA in the GnIH orexigenic response and NPY, POMC and MCH are likely also involved.",
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    AU - Gilbert, Elizabeth R.

    AU - Osugi, Tomohiro

    AU - Ubuka, Takayoshi

    AU - Tsutsui, Kazuyoshi

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    AU - Furuse, Mitsuhiro

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