Green tea epigalocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via inhibition of both FAK and IGF-1R

H. A. Vu, Y. Beppu, H. T. Chi, K. Sasaki, H. Yamamoto, P. T. Xinh, T. Tanii, Y. Hara, T. Watanabe, Y. Sato, I. Ohdomari

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Objectives: Green tea epigalocatechin galate (EGCG) has been shown to exhibit a growth-suppressive effect on human pancreatic cancer cells; however, the exact molecular mechanism by which EGCG suppresses cell proliferation is unclear. We hypothesize that interference with cell adhesion might be one mechanism. Methods: The effect of EGCG on cell adhesion and proliferation was examined using pancreatic cancer cells. Results: EGCG-treated pancreatic cancer cells AsPC-1 and BxPC-3 decrease cell adhesion ability on micro-pattern dots, accompanied by dephosphorylations of both focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), whereas retained the activations of mitogen-activated protein kinase and mammalian target of rapamycin. The growth of AsPC-1 and BxPC-3 cells, when cultured at low density, can be significantly suppressed by EGCG treatment alone in a dose-dependent manner. At a dose of 100 μM that completely abolishes activations of FAK and IGF-1R, EGCG suppresses more than 50% of cell proliferation without evidence of apoptosis analyzed by PARP cleavage. Finally, the MEK1/2 inhibitor U0126 enhances growth-suppressive effect of EGCG. Conclusions: Blocking FAK and IGF-1R by EGCG could prove valuable for targeted therapy, which can use in combination with other therapies, for pancreatic cancer.

Original languageEnglish
Title of host publicationThe Third International Conference on the Development of Biomedical Engineering in Vietnam
Pages223-226
Number of pages4
DOIs
Publication statusPublished - 2010 May 21
Event3rd International Conference on the Development of Biomedical Engineering in Vietnam - Ho Chi Minh City, Viet Nam
Duration: 2010 Jan 112010 Jan 14

Publication series

NameIFMBE Proceedings
Volume27
ISSN (Print)1680-0737

Other

Other3rd International Conference on the Development of Biomedical Engineering in Vietnam
CountryViet Nam
CityHo Chi Minh City
Period10/1/1110/1/14

Keywords

  • EGCG
  • FAK
  • IGF-1R
  • N-cadherin
  • Pancreatic carcinoma

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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    Vu, H. A., Beppu, Y., Chi, H. T., Sasaki, K., Yamamoto, H., Xinh, P. T., Tanii, T., Hara, Y., Watanabe, T., Sato, Y., & Ohdomari, I. (2010). Green tea epigalocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via inhibition of both FAK and IGF-1R. In The Third International Conference on the Development of Biomedical Engineering in Vietnam (pp. 223-226). (IFMBE Proceedings; Vol. 27). https://doi.org/10.1007/978-3-642-12020-6-56