Green tea epigalocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via inhibition of both FAK and IGF-1R

H. A. Vu, Y. Beppu, H. T. Chi, K. Sasaki, H. Yamamoto, P. T. Xinh, Takashi Tanii, Y. Hara, T. Watanabe, Y. Sato, I. Ohdomari

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    Abstract

    Objectives: Green tea epigalocatechin galate (EGCG) has been shown to exhibit a growth-suppressive effect on human pancreatic cancer cells; however, the exact molecular mechanism by which EGCG suppresses cell proliferation is unclear. We hypothesize that interference with cell adhesion might be one mechanism. Methods: The effect of EGCG on cell adhesion and proliferation was examined using pancreatic cancer cells. Results: EGCG-treated pancreatic cancer cells AsPC-1 and BxPC-3 decrease cell adhesion ability on micro-pattern dots, accompanied by dephosphorylations of both focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), whereas retained the activations of mitogen-activated protein kinase and mammalian target of rapamycin. The growth of AsPC-1 and BxPC-3 cells, when cultured at low density, can be significantly suppressed by EGCG treatment alone in a dose-dependent manner. At a dose of 100 μM that completely abolishes activations of FAK and IGF-1R, EGCG suppresses more than 50% of cell proliferation without evidence of apoptosis analyzed by PARP cleavage. Finally, the MEK1/2 inhibitor U0126 enhances growth-suppressive effect of EGCG. Conclusions: Blocking FAK and IGF-1R by EGCG could prove valuable for targeted therapy, which can use in combination with other therapies, for pancreatic cancer.

    Original languageEnglish
    Title of host publicationIFMBE Proceedings
    Pages223-226
    Number of pages4
    Volume27
    DOIs
    Publication statusPublished - 2010
    Event3rd International Conference on the Development of Biomedical Engineering in Vietnam - Ho Chi Minh City
    Duration: 2010 Jan 112010 Jan 14

    Other

    Other3rd International Conference on the Development of Biomedical Engineering in Vietnam
    CityHo Chi Minh City
    Period10/1/1110/1/14

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    Keywords

    • EGCG
    • FAK
    • IGF-1R
    • N-cadherin
    • Pancreatic carcinoma

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering

    Cite this

    Vu, H. A., Beppu, Y., Chi, H. T., Sasaki, K., Yamamoto, H., Xinh, P. T., Tanii, T., Hara, Y., Watanabe, T., Sato, Y., & Ohdomari, I. (2010). Green tea epigalocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via inhibition of both FAK and IGF-1R. In IFMBE Proceedings (Vol. 27, pp. 223-226) https://doi.org/10.1007/978-3-642-12020-6-56