Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor

Kazuichi Isaka, Yasuhiro Date, Tatsuo Sumino, Sachiko Yoshie, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    112 Citations (Scopus)

    Abstract

    The doubling time of anaerobic ammonium-oxidizing (anammox) bacteria in an anaerobic biological filtrated (ABF) reactor was determined. Fluorescence in situ hybridization analysis was used to detect and count anammox bacteria cells in anammox sludge. As a result, the populations of anammox bacteria at 14th and 21st days were 1.1×106 and 1.7×107 cells/ml reactor, respectively. From these results, the doubling time of anammox bacteria was calculated as 1.8 days, and the specific growth rate (μ) was 0.39 day-1. This result indicated that the anammox bacteria have higher growth rate than the reported value (doubling time, 11 days). Furthermore, it was clearly demonstrated that nitrogen conversion rate was proportional to the population of anammox bacteria. Maintaining the ideal environment for the growth of anammox bacteria in the ABF reactor might lead to faster growth. This is the first report of the growth rate of anammox bacteria based on the direct counting of anammox bacteria.

    Original languageEnglish
    Pages (from-to)47-52
    Number of pages6
    JournalApplied Microbiology and Biotechnology
    Volume70
    Issue number1
    DOIs
    Publication statusPublished - 2006 Mar

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    Anaerobic Bacteria
    Ammonium Compounds
    Bacteria
    Growth
    Sewage
    Fluorescence In Situ Hybridization
    Population
    Fluorescence
    Nitrogen

    ASJC Scopus subject areas

    • Biotechnology
    • Microbiology
    • Bioengineering
    • Microbiology (medical)

    Cite this

    Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor. / Isaka, Kazuichi; Date, Yasuhiro; Sumino, Tatsuo; Yoshie, Sachiko; Tsuneda, Satoshi.

    In: Applied Microbiology and Biotechnology, Vol. 70, No. 1, 03.2006, p. 47-52.

    Research output: Contribution to journalArticle

    Isaka, Kazuichi ; Date, Yasuhiro ; Sumino, Tatsuo ; Yoshie, Sachiko ; Tsuneda, Satoshi. / Growth characteristic of anaerobic ammonium-oxidizing bacteria in an anaerobic biological filtrated reactor. In: Applied Microbiology and Biotechnology. 2006 ; Vol. 70, No. 1. pp. 47-52.
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