Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure

Sumio Iwai, Sho Ogata, Naotaka Yamada, Michio Onjo, Kintake Sonoike, Ken ichiro Shimazaki

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.

Original languageEnglish
Article numbere00137
JournalPlant Direct
Volume3
Issue number5
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

Abscisic Acid
abscisic acid
Photosynthesis
guard cells
reactive oxygen species
Reactive Oxygen Species
photosynthesis
Electron Transport
electron transfer
NADPH Oxidase
Chloroplasts
chloroplast
inhibitor
electron
chloroplasts
Dibromothymoquinone
Commelina
Commelina benghalensis
Diuron
Physiological Phenomena

Keywords

  • abscisic acid
  • Arabidopsis thaliana
  • Commelina benghalensis
  • guard cell photosynthesis
  • reactive oxygen species
  • Vicia faba

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

Cite this

Iwai, S., Ogata, S., Yamada, N., Onjo, M., Sonoike, K., & Shimazaki, K. I. (2019). Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. Plant Direct, 3(5), [e00137]. https://doi.org/10.1002/pld3.137

Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. / Iwai, Sumio; Ogata, Sho; Yamada, Naotaka; Onjo, Michio; Sonoike, Kintake; Shimazaki, Ken ichiro.

In: Plant Direct, Vol. 3, No. 5, e00137, 01.05.2019.

Research output: Contribution to journalArticle

Iwai, Sumio ; Ogata, Sho ; Yamada, Naotaka ; Onjo, Michio ; Sonoike, Kintake ; Shimazaki, Ken ichiro. / Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure. In: Plant Direct. 2019 ; Vol. 3, No. 5.
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