Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure

Sumio Iwai, Sho Ogata, Naotaka Yamada, Michio Onjo, Kintake Sonoike, Ken ichiro Shimazaki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.

Original languageEnglish
Article numbere00137
JournalPlant Direct
Volume3
Issue number5
DOIs
Publication statusPublished - 2019 May

Keywords

  • Arabidopsis thaliana
  • Commelina benghalensis
  • Vicia faba
  • abscisic acid
  • guard cell photosynthesis
  • reactive oxygen species

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Plant Science

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