Abstract
Previously, we found that a small (approx. 20-mer) DNA hybridizing to the 5′-leader region of a tRNA precursor enhances the cleavage efficiency in bacterial ribonuclease P reaction. We named this technique the 'guide DNA technique'. Detailed analyses showed that the length of the guide DNA, concentration of the guide DNA and the hybridizing position affected the cleavage efficiency: for an effective cleavage reaction, guide DNA should be designed to hybridize to the region on the cleavage site, should be 20 bases or more in length and should be of high concentration. The presence of a 5′-flanking region in the DNA did not affect the cleavage reaction. The guide DNA technique is a useful tool for effective preparation of mature tRNA molecules in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 85-88 |
| Number of pages | 4 |
| Journal | Biotechnology and Applied Biochemistry |
| Volume | 36 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2002 Oct |
| Externally published | Yes |
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Keywords
- Escherichia coli
- Leader sequence
- Maturation
- Ribozyme
- RNase P
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
Cite this
Guide DNA technique in bacterial ribonuclease P reaction for effective processing of tRNA precursor. / Tanaka, Terumichi; Hori, Yoshiaki; Kikuchi, Yo.
In: Biotechnology and Applied Biochemistry, Vol. 36, No. 2, 10.2002, p. 85-88.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Guide DNA technique in bacterial ribonuclease P reaction for effective processing of tRNA precursor
AU - Tanaka, Terumichi
AU - Hori, Yoshiaki
AU - Kikuchi, Yo
PY - 2002/10
Y1 - 2002/10
N2 - Previously, we found that a small (approx. 20-mer) DNA hybridizing to the 5′-leader region of a tRNA precursor enhances the cleavage efficiency in bacterial ribonuclease P reaction. We named this technique the 'guide DNA technique'. Detailed analyses showed that the length of the guide DNA, concentration of the guide DNA and the hybridizing position affected the cleavage efficiency: for an effective cleavage reaction, guide DNA should be designed to hybridize to the region on the cleavage site, should be 20 bases or more in length and should be of high concentration. The presence of a 5′-flanking region in the DNA did not affect the cleavage reaction. The guide DNA technique is a useful tool for effective preparation of mature tRNA molecules in vitro.
AB - Previously, we found that a small (approx. 20-mer) DNA hybridizing to the 5′-leader region of a tRNA precursor enhances the cleavage efficiency in bacterial ribonuclease P reaction. We named this technique the 'guide DNA technique'. Detailed analyses showed that the length of the guide DNA, concentration of the guide DNA and the hybridizing position affected the cleavage efficiency: for an effective cleavage reaction, guide DNA should be designed to hybridize to the region on the cleavage site, should be 20 bases or more in length and should be of high concentration. The presence of a 5′-flanking region in the DNA did not affect the cleavage reaction. The guide DNA technique is a useful tool for effective preparation of mature tRNA molecules in vitro.
KW - Escherichia coli
KW - Leader sequence
KW - Maturation
KW - Ribozyme
KW - RNase P
UR - http://www.scopus.com/inward/record.url?scp=0036794378&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036794378&partnerID=8YFLogxK
U2 - 10.1042/BA20020047
DO - 10.1042/BA20020047
M3 - Article
VL - 36
SP - 85
EP - 88
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
SN - 0885-4513
IS - 2
ER -