Gut Microbiota-Derived Short Chain Fatty Acids Induce Circadian Clock Entrainment in Mouse Peripheral Tissue

Yu Tahara, Mayu Yamazaki, Haruna Sukigara, Hiroaki Motohashi, Hiroyuki Sasaki, Hiroki Miyakawa, Atsushi Haraguchi, Yuko Ikeda, Shinji Fukuda, Shigenobu Shibata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Microbiota-derived short-chain fatty acids (SCFAs) and organic acids produced by the fermentation of non-digestible fibre can communicate from the microbiome to host tissues and modulate homeostasis in mammals. The microbiome has circadian rhythmicity and helps the host circadian clock function. We investigated the effect of SCFA or fibre-containing diets on circadian clock phase adjustment in mouse peripheral tissues (liver, kidney, and submandibular gland). Initially, caecal SCFA concentrations, particularly acetate and butyrate, induced significant day-night differences at high concentrations during the active period, which were correlated with lower caecal pH. By monitoring luciferase activity correlated with the clock gene Period2 in vivo, we found that oral administration of mixed SCFA (acetate, butyrate, and propionate) and an organic acid (lactate), or single administration of each SCFA or lactate for three days, caused phase changes in the peripheral clocks with stimulation timing dependency. However, this effect was not detected in cultured fibroblasts or cultured liver slices with SCFA applied to the culture medium, suggesting SCFA-induced indirect modulation of circadian clocks in vivo. Finally, cellobiose-containing diets facilitated SCFA production and refeeding-induced peripheral clock entrainment. SCFA oral gavage and prebiotic supplementation can facilitate peripheral clock adjustment, suggesting prebiotics as novel therapeutic candidates for misalignment.

Original languageEnglish
Article number1395
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Circadian Clocks
Volatile Fatty Acids
Microbiota
Prebiotics
Butyrates
Lactic Acid
Acetates
Diet
Gastrointestinal Microbiome
Cellobiose
Acids
Submandibular Gland
Liver
Propionates
Periodicity
Luciferases
Fermentation
Oral Administration
Culture Media
Mammals

ASJC Scopus subject areas

  • General

Cite this

Gut Microbiota-Derived Short Chain Fatty Acids Induce Circadian Clock Entrainment in Mouse Peripheral Tissue. / Tahara, Yu; Yamazaki, Mayu; Sukigara, Haruna; Motohashi, Hiroaki; Sasaki, Hiroyuki; Miyakawa, Hiroki; Haraguchi, Atsushi; Ikeda, Yuko; Fukuda, Shinji; Shibata, Shigenobu.

In: Scientific Reports, Vol. 8, No. 1, 1395, 01.12.2018.

Research output: Contribution to journalArticle

Tahara, Yu ; Yamazaki, Mayu ; Sukigara, Haruna ; Motohashi, Hiroaki ; Sasaki, Hiroyuki ; Miyakawa, Hiroki ; Haraguchi, Atsushi ; Ikeda, Yuko ; Fukuda, Shinji ; Shibata, Shigenobu. / Gut Microbiota-Derived Short Chain Fatty Acids Induce Circadian Clock Entrainment in Mouse Peripheral Tissue. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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