Gut pathobionts underlie intestinal barrier dysfunction and liver T helper 17 cell immune response in primary sclerosing cholangitis

Nobuhiro Nakamoto, Nobuo Sasaki, Ryo Aoki, Kentaro Miyamoto, Wataru Suda, Toshiaki Teratani, Takahiro Suzuki, Yuzo Koda, Po Sung Chu, Nobuhito Taniki, Akihiro Yamaguchi, Mitsuhiro Kanamori, Nobuhiko Kamada, Masahira Hattori, Hiroshi Ashida, Michiie Sakamoto, Koji Atarashi, Seiko Narushima, Akihiko Yoshimura, Kenya HondaToshiro Sato, Takanori Kanai

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Primary sclerosing cholangitis (PSC) is a chronic inflammatory liver disease and its frequent complication with ulcerative colitis highlights the pathogenic role of epithelial barrier dysfunction. Intestinal barrier dysfunction has been implicated in the pathogenesis of PSC, yet its underlying mechanism remains unknown. Here, we identify Klebsiella pneumonia in the microbiota of patients with PSC and demonstrate that K. pneumoniae disrupts the epithelial barrier to initiate bacterial translocation and liver inflammatory responses. Gnotobiotic mice inoculated with PSC-derived microbiota exhibited T helper 17 (TH17) cell responses in the liver and increased susceptibility to hepatobiliary injuries. Bacterial culture of mesenteric lymph nodes in these mice isolated K. pneumoniae, Proteus mirabilis and Enterococcus gallinarum, which were prevalently detected in patients with PSC. A bacterial-organoid co-culture system visualized the epithelial-damaging effect of PSC-derived K. pneumoniae that was associated with bacterial translocation and susceptibility to TH17-mediated hepatobiliary injuries. We also show that antibiotic treatment ameliorated the TH17 immune response induced by PSC-derived microbiota. These results highlight the role of pathobionts in intestinal barrier dysfunction and liver inflammation, providing insights into therapeutic strategies for PSC.

    Original languageEnglish
    JournalNature Microbiology
    DOIs
    Publication statusAccepted/In press - 2019 Jan 1

    Fingerprint

    Th17 Cells
    Sclerosing Cholangitis
    Liver Diseases
    Microbiota
    Bacterial Translocation
    Pneumonia
    Organoids
    Germ-Free Life
    Proteus mirabilis
    Liver
    Enterococcus
    Wounds and Injuries
    Klebsiella pneumoniae
    Coculture Techniques
    Ulcerative Colitis
    Lymph Nodes
    Anti-Bacterial Agents
    Inflammation
    Therapeutics

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Applied Microbiology and Biotechnology
    • Genetics
    • Microbiology (medical)
    • Cell Biology

    Cite this

    Gut pathobionts underlie intestinal barrier dysfunction and liver T helper 17 cell immune response in primary sclerosing cholangitis. / Nakamoto, Nobuhiro; Sasaki, Nobuo; Aoki, Ryo; Miyamoto, Kentaro; Suda, Wataru; Teratani, Toshiaki; Suzuki, Takahiro; Koda, Yuzo; Chu, Po Sung; Taniki, Nobuhito; Yamaguchi, Akihiro; Kanamori, Mitsuhiro; Kamada, Nobuhiko; Hattori, Masahira; Ashida, Hiroshi; Sakamoto, Michiie; Atarashi, Koji; Narushima, Seiko; Yoshimura, Akihiko; Honda, Kenya; Sato, Toshiro; Kanai, Takanori.

    In: Nature Microbiology, 01.01.2019.

    Research output: Contribution to journalArticle

    Nakamoto, N, Sasaki, N, Aoki, R, Miyamoto, K, Suda, W, Teratani, T, Suzuki, T, Koda, Y, Chu, PS, Taniki, N, Yamaguchi, A, Kanamori, M, Kamada, N, Hattori, M, Ashida, H, Sakamoto, M, Atarashi, K, Narushima, S, Yoshimura, A, Honda, K, Sato, T & Kanai, T 2019, 'Gut pathobionts underlie intestinal barrier dysfunction and liver T helper 17 cell immune response in primary sclerosing cholangitis', Nature Microbiology. https://doi.org/10.1038/s41564-018-0333-1
    Nakamoto, Nobuhiro ; Sasaki, Nobuo ; Aoki, Ryo ; Miyamoto, Kentaro ; Suda, Wataru ; Teratani, Toshiaki ; Suzuki, Takahiro ; Koda, Yuzo ; Chu, Po Sung ; Taniki, Nobuhito ; Yamaguchi, Akihiro ; Kanamori, Mitsuhiro ; Kamada, Nobuhiko ; Hattori, Masahira ; Ashida, Hiroshi ; Sakamoto, Michiie ; Atarashi, Koji ; Narushima, Seiko ; Yoshimura, Akihiko ; Honda, Kenya ; Sato, Toshiro ; Kanai, Takanori. / Gut pathobionts underlie intestinal barrier dysfunction and liver T helper 17 cell immune response in primary sclerosing cholangitis. In: Nature Microbiology. 2019.
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