Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo

Yosuke Okamura; Toshinori Fujie; Makoto Handa; Yasuo Ikeda; Shinji Takeoka

Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo

Yosuke Okamura^*, Toshinori Fujie, Makoto Handa, Yasuo Ikeda, Shinji Takeoka

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

We have studied prototypes of platelet substitutes and focused on the dodecapeptide (H12); a part of the amino acid sequence of fibrinogen as activated platelets, and conjugated H12 to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles. The H12-conjugated polyAlb (H12-polyAlb) adding to the thrombocytopenic-imitation blood enhanced the platelet thrombus formation on a collagen-plate in vitro. We confirmed that H12-polyAlb had a hemostatic effect, based on the analysis of the tail bleeding time of thrombocytopenic rats. H12-polyAlb is a suitable candidate for an alternative to human platelet concentrates infused into thrombocytopenic patients.

Original language	English
Pages	4933-4934
Number of pages	2
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sept 20 → 2005 Sept 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

Fibrinogen γ chain dodecapeptide (H12)
Flow conditions
Haemostatic effects
Platelet substitutes
Polymerized albumin particles (polyAlb)
Thrombocytopenic rats

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo",

abstract = "We have studied prototypes of platelet substitutes and focused on the dodecapeptide (H12); a part of the amino acid sequence of fibrinogen as activated platelets, and conjugated H12 to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles. The H12-conjugated polyAlb (H12-polyAlb) adding to the thrombocytopenic-imitation blood enhanced the platelet thrombus formation on a collagen-plate in vitro. We confirmed that H12-polyAlb had a hemostatic effect, based on the analysis of the tail bleeding time of thrombocytopenic rats. H12-polyAlb is a suitable candidate for an alternative to human platelet concentrates infused into thrombocytopenic patients.",

keywords = "Fibrinogen γ chain dodecapeptide (H12), Flow conditions, Haemostatic effects, Platelet substitutes, Polymerized albumin particles (polyAlb), Thrombocytopenic rats",

author = "Yosuke Okamura and Toshinori Fujie and Makoto Handa and Yasuo Ikeda and Shinji Takeoka",

year = "2005",

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day = "1",

language = "English",

pages = "4933--4934",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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T1 - Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo

AU - Okamura, Yosuke

AU - Fujie, Toshinori

AU - Handa, Makoto

AU - Ikeda, Yasuo

AU - Takeoka, Shinji

PY - 2005/12/1

Y1 - 2005/12/1

N2 - We have studied prototypes of platelet substitutes and focused on the dodecapeptide (H12); a part of the amino acid sequence of fibrinogen as activated platelets, and conjugated H12 to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles. The H12-conjugated polyAlb (H12-polyAlb) adding to the thrombocytopenic-imitation blood enhanced the platelet thrombus formation on a collagen-plate in vitro. We confirmed that H12-polyAlb had a hemostatic effect, based on the analysis of the tail bleeding time of thrombocytopenic rats. H12-polyAlb is a suitable candidate for an alternative to human platelet concentrates infused into thrombocytopenic patients.

AB - We have studied prototypes of platelet substitutes and focused on the dodecapeptide (H12); a part of the amino acid sequence of fibrinogen as activated platelets, and conjugated H12 to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles. The H12-conjugated polyAlb (H12-polyAlb) adding to the thrombocytopenic-imitation blood enhanced the platelet thrombus formation on a collagen-plate in vitro. We confirmed that H12-polyAlb had a hemostatic effect, based on the analysis of the tail bleeding time of thrombocytopenic rats. H12-polyAlb is a suitable candidate for an alternative to human platelet concentrates infused into thrombocytopenic patients.

KW - Fibrinogen γ chain dodecapeptide (H12)

KW - Flow conditions

KW - Haemostatic effects

KW - Platelet substitutes

KW - Polymerized albumin particles (polyAlb)

KW - Thrombocytopenic rats

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M3 - Paper

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T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo

Abstract

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Keywords

ASJC Scopus subject areas

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