Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo

Yosuke Okamura, Toshinori Fujie, Makoto Handa, Yasuo Ikeda, Shinji Takeoka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have studied prototypes of platelet substitutes and focused on the dodecapeptide (H12); a part of the amino acid sequence of fibrinogen as activated platelets, and conjugated H12 to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles. The H12-conjugated polyAlb (H12-polyAlb) adding to the thrombocytopenic-imitation blood enhanced the platelet thrombus formation on a collagen-plate in vitro. We confirmed that H12-polyAlb had a hemostatic effect, based on the analysis of the tail bleeding time of thrombocytopenic rats. H12-polyAlb is a suitable candidate for an alternative to human platelet concentrates infused into thrombocytopenic patients.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages4933-4934
Number of pages2
Volume54
Edition2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Fingerprint

Platelets
Collagen
Amino acids
Rats
Blood

Keywords

  • Fibrinogen γ chain dodecapeptide (H12)
  • Flow conditions
  • Haemostatic effects
  • Platelet substitutes
  • Polymerized albumin particles (polyAlb)
  • Thrombocytopenic rats

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Okamura, Y., Fujie, T., Handa, M., Ikeda, Y., & Takeoka, S. (2005). Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo. In Polymer Preprints, Japan (2 ed., Vol. 54, pp. 4933-4934)

Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo. / Okamura, Yosuke; Fujie, Toshinori; Handa, Makoto; Ikeda, Yasuo; Takeoka, Shinji.

Polymer Preprints, Japan. Vol. 54 2. ed. 2005. p. 4933-4934.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okamura, Y, Fujie, T, Handa, M, Ikeda, Y & Takeoka, S 2005, Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo. in Polymer Preprints, Japan. 2 edn, vol. 54, pp. 4933-4934, 54th SPSJ Symposium on Macromolecules, Yamagata, Japan, 05/9/20.
Okamura Y, Fujie T, Handa M, Ikeda Y, Takeoka S. Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo. In Polymer Preprints, Japan. 2 ed. Vol. 54. 2005. p. 4933-4934
Okamura, Yosuke ; Fujie, Toshinori ; Handa, Makoto ; Ikeda, Yasuo ; Takeoka, Shinji. / Haemostatic effects of polymerized albumin particles carrying fibrinogen-γ chain dodecapeptide as platelet substitutes in vitro and in vivo. Polymer Preprints, Japan. Vol. 54 2. ed. 2005. pp. 4933-4934
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