HDAC activity is required for BDNF to increase quantal neurotransmitter release and dendritic spine density in CA1 pyramidal neurons

Gaston Calfa, Christopher A. Chapleau, Susan Campbell, Takafumi Inoue, Sarah J. Morse, Farah D. Lubin, Lucas Pozzo-Miller

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Molecular mechanisms involved in the strengthening and formation of synapses include the activation and repression of specific genes or subsets of genes by epigenetic modifications that do not alter the genetic code itself. Chromatin modifications mediated by histone acetylation have been shown to be critical for synaptic plasticity at hippocampal excitatory synapses and hippocampal-dependent memory formation. Considering that brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity and behavioral adaptations, it is not surprising that regulation of this gene is subject to histone acetylation changes during synaptic plasticity and hippocampal-dependent memory formation. Whether the effects of BDNF on dendritic spines and quantal transmitter release require histone modifications remains less known. By using two different inhibitors of histone deacetylases (HDACs), we describe here that their activity is required for BDNF to increase dendritic spine density and excitatory quantal transmitter release onto CA1 pyramidal neurons in hippocampal slice cultures. These results suggest that histone acetylation/deacetylation is a critical step in the modulation of hippocampal synapses by BDNF. Thus, mechanisms ofepigenetic modulation of synapse formation and function are novel targets to consider for the amelioration of symptoms of intellectual disabilities and neurodegenerative disorders associated with cognitive and memory deficits.

    Original languageEnglish
    Pages (from-to)1493-1500
    Number of pages8
    JournalHippocampus
    Volume22
    Issue number7
    DOIs
    Publication statusPublished - 2012 Jul

    Fingerprint

    Dendritic Spines
    Histone Deacetylases
    Pyramidal Cells
    Brain-Derived Neurotrophic Factor
    Synapses
    Neuronal Plasticity
    Neurotransmitter Agents
    Acetylation
    Histone Code
    Histones
    Genes
    Genetic Code
    Memory Disorders
    Epigenomics
    Intellectual Disability
    Neurodegenerative Diseases
    Chromatin

    Keywords

    • BDNF
    • Dendritic spines
    • Epigenetics
    • Hippocampal slice cultures
    • Histone deacetylase
    • Quantal release
    • SAHA
    • TSA

    ASJC Scopus subject areas

    • Cognitive Neuroscience

    Cite this

    HDAC activity is required for BDNF to increase quantal neurotransmitter release and dendritic spine density in CA1 pyramidal neurons. / Calfa, Gaston; Chapleau, Christopher A.; Campbell, Susan; Inoue, Takafumi; Morse, Sarah J.; Lubin, Farah D.; Pozzo-Miller, Lucas.

    In: Hippocampus, Vol. 22, No. 7, 07.2012, p. 1493-1500.

    Research output: Contribution to journalArticle

    Calfa, Gaston ; Chapleau, Christopher A. ; Campbell, Susan ; Inoue, Takafumi ; Morse, Sarah J. ; Lubin, Farah D. ; Pozzo-Miller, Lucas. / HDAC activity is required for BDNF to increase quantal neurotransmitter release and dendritic spine density in CA1 pyramidal neurons. In: Hippocampus. 2012 ; Vol. 22, No. 7. pp. 1493-1500.
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